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W. D. HALLIBURTON. 



hour; the corpuscles settle at the bottom of the tube, and 

 the supernatant serum is pipetted off. To the corpuscles the 

 blood-serum of some other animal is added, the mixture shaken, 

 and the mixture again centrifugalised ; the serum is again 

 pipetted off, and more added. After repeating this process 

 several times, the corpuscles of one animal are obtained in the 

 serum of another animal without any of the serum of the first 

 animal being in the mixture. Haemoglobin crystals are then 

 prepared from this mixture. In some cases the foreign serum 

 dissolves the haemoglobin and disintegrates the corpuscles. 

 This was first pointed out by Landois. 1 



Mere addition of the blood-serum of one animal does not 

 as a rule cause the formation of blood-crystals. It does so, 

 however, sometimes. 2 This is explicable on the assumption 

 that the blood-serum used is very watery, and the haemoglobin 

 of the other animal crystallises very readily. I have myself 

 come across no case in which it occurred. 



My results may be best given in the form of the following 

 table. I have given not only the effect of the foreign serum on the 

 crystalline form of haemoglobin, but also the effect on the cor- 

 puscles themselves, as to whether they are disintegrated or not. 



The result of these experiments is to show that the serum of 

 one animal has no influence in causing a change of the haemo- 

 globin crystals of another animal. 



I next examined in a qualitative manner the serum of certain 



1 ' Die Transfusion des Blutes,' Leipzig, 1874. 



2 An instance of such action is recorded by Professor Scbafer (" Blood 

 Transfusion," ' Trans. Obst. Soc. London,' 1879, p. 317). 



