HEMOGLOBIN CRYSTALS OF RODENTS BLOOD. 



193 



rodents with regard to the proteids or albuminous substances 

 contained in it. I obtained similar results in all animals, 

 results which show, too, that the serum proteids of rodents 

 agree with those in other mammalian animals which I had 

 previously investigated. 1 The proteids, the most important 

 bodies in the blood-plasma, being similar, the serum would 

 not on a priori grounds be suspected of influencing the 

 crystalline form of haemoglobin. The results I have obtained 

 with regard to the heat- coagulation temperatures of these 

 bodies is shown in the following table. 



Temperatures of Coagulation of the Proteids in the 

 Blood of certain Rodents. 



Name of Proteid. 



Globulins — 



Fibrinogen . . 

 Serum globulins 



Albumins — 



Guinea-pig. 



Squirrel. 



5G° 

 75° 



73° | 70° - 70°-l° 72° 



76° 77° 78° 77° 



C. 



C. 



56° 



75° 



72°-3° 



C 



5f>° 

 75° 



73° 



7 ; (small in amount) 77° 



84° j 84° I 84° 87° (trace) S-t (very abundant) 84°| 



The stromata of the red blood-corpuscles might, however, 

 possibly be supposed to have some influence on the crystalline 

 form of the haemoglobin. We have seen that crystallising the 

 haemoglobin of one animal from the serum of another yielded 

 negative results ; squirrel's haemoglobin remained hexagonal, 

 rat's and guinea-pig's rhombic prisms and tetrahedra respec- 

 tively, whatever the serum in which they had been dissolved. 

 A similar result followed crystallisation from a fluid consisting 

 of serum plus the dissolved stromata of the corpuscles of some 

 other animal. This was obtained by adding to the blood one 

 sixteenth of its volume of ether, and letting it stand ; the 

 crystals of haemoglobin which formed were filtered off, and the 

 ether evaporated from the filtrate which consisted of the serum 

 with the stromata of the corpuscles dissolved in it. 



1 Halliburton, "Periods of Serum," ' Journal of Physiology,' vol. v, p. 152. 



VOL. XXVIII, PART 1. NEW SER. N 



