140 SIDNEY F. HARMER. 
on all the polypide buds which were in their critical state im- 
mediately preceding fusion in such a way as to prevent fusion, 
and so to cause each half to develop into a complete bud. 
In a few cases which appeared to be abnormal the young 
bud made its first appearance in close contact with the “ brown 
body.” Buds developed in this position differed from the 
normal buds in being more spherical at a stage when the 
tentacles were first seen, and in some other respects. The 
differences subsequently became less marked, and the bud 
appeared to develop into a normal polypide, although my time 
was not sufficient to allow me to watch the last stages in this 
process. 
At the growing edge of the colony the polypide-buds are 
formed at the extreme proximal edge of the zocecia. 
The development of the young bud into a complete polypide 
may be considered in connexion with the indigo-carmine ex- 
periments, the behaviour of the developing buds being, in 
those experiments, exactly similar to that of the bud developing 
under normal circumstances. 
The zooecia are found, after the action of indigo-carmine, to 
have a brilliant blue colour owing to the absorption of the 
pigment by the leucocytes, as already described. Some of the 
zocecia may be dead, and are then readily recognised by the 
fact that they are stained intensely, but diffusely, blue, the 
operculum being usually open. In these zocecia, Infusoria 
and Nematodes soon make their appearance. 
In zoccia which are still alive the pigment is confined to 
the cells already described. The leucocytes after several days 
tend to group themselves in masses, and some of them may be 
seen closely surrounding the “ brown bodies ” (fig. 1). 
About 114 hours after the commencement of the experiment 
which turned out most successfully, all the functional polypides 
of the colony were noticed to be degenerating, the evidence of 
which ‘was given by the fact that the feces had ceased to 
rotate in the intestine. At the 165th hour the indigo-carmine 
taken up by the two groups of granules in the wall of the 
alimentary canal was, in many cases, becoming aggregated 
