150 SIDNEY F. HARMER. 
The amount of pigment in the funicular tissue had con- 
siderably diminished by the eighth day (170 hours), probably 
indicating that it had been used for the nourishment of the 
young polypide, a view which is confirmed by the facts that 
the bud is invariably surrounded by a dense network of funi- 
cular tissue, and that the tissue in question is very largely 
developed at the growing-points. By the end of the same day 
the cecum of the young polypide projected considerably be- 
yond the proximal side of the “ brown body,” and one or two 
of the blue leucocytes had passed into the ring-canals of the 
young polypides. 
At the eleventh day (243 hours) the polypides had reached the 
stage shown for Flustra in fig. 4 (sixteenth day). The“ brown 
body” was fully formed and the aperture was in process of 
development. The funicular tissue appeared to be appre- 
ciably diminished in amount, and had lost most of its granules. 
Although some of these granules are probably used for the 
nutrition of the young polypide, others appear to give rise to 
the spherules of brown matter seen in various parts of the 
body-cavity. These spherules may be regarded as effete por- 
tions of the funicular tissue which are deposited, with the 
“brown body”? itself, in the body-cavity. 
At the end of the fourteenth day (288 hours) the funicular 
tissue was regaining its purple colour, which had been lost 
during the occurrence of the processes already described. I am 
inclined to regard this purple pigment as being of nutritive 
rather than of excretory value. 
Fig. 17 shows the condition of the polypides regenerated since 
the beginning of the experiment at the fourteenth day (316 
hours). The polypide had by this time become very irritable, 
but was apparently not yet capable of protrusion. No essen- 
tial change had taken place in the leucocytes, which were still 
bright blue. In the individual shown in the figure a large 
mass of these leucocytes occurred at the distal end of the 
zocecium. Twenty-four hours later food was circulating in 
the intestine, and the aperture appeared to be completely 
formed. 
