174 J. T. CUNNINGHAM. 
of sections prepared, since my retnrn, from material preserved 
in Norway. The testicular tissue was preserved with Flem- 
ming’s mixture of chromic, osmic, and acetic acids. I have 
found the best staining liquid to be an aqueous solution of 
saffranin. In all the pieces of testis which I have cut into 
sections I have found that only a small number of the capsules 
contain ripe spermatozoa. A great many capsules are always 
full of cells containing large nuclei in the resting condition : 
examples of these cells are represented in Plate IV, fig. 4. 
Other capsules are full of similar cells preparing to divide, the 
chromatin of the nuclei being in the skein form, as shown in 
fig. 5 of the same plate. In other capsules, again, cells of the 
same large size exhibit the stage of the equatorial plate, as in 
fig. 6, the chromatin being in the form of a number of densely 
stained rods at the equator of a protoplasmic spindle. In my 
preparations the capsules in the last condition are not full, the 
cells being separate from one another, not in contact as in other 
cases. 
The spermatozoa are seen with other elements in a few 
capsules of a section. Figs. 7, 8, and 9 exhibit the cellular 
elements seen in a single ripe capsule in onesection. In fig. 9 
are shown one perfect spermatozoon, several spindle-shaped 
cells, and one other structure. The spindle @ is destitute of 
chromatin: it has an axial core more deeply stained than the 
rest of the cell, and continuous with the attenuated processes. 
This core consists of very faint longitudinal striez. One of 
the processes in the spindle 4 is connected with a sperm- 
nucleus, but there is no chromatin in the body of the spindle ; 
the axial core is distinct. At c is seen another spindle in 
which the core is not distinguishable, and in which there is a 
dense small nucleus similar in appearance to a sperm-nucleus, 
but more rounded in shape. At d is a smaller spindle very 
faintly stained, containing a small nucleus, whose structure 1s 
that of an ordinary resting nucleus and not of a sperm-nucleus. 
g is a spindle in which a sperm-nucleus is situated at the 
base of one of the processes ; / is similar to c, except that the 
core is visible as well as the nuclear body. Comparison of 
