222, CHARLES SLATER. 
The cells contain numerous vacuoles apparently filled with 
colloidal material, and the web of granular material passing 
amongst the cells of the leprous granulomata seems to form 
the boundary of similar vacuoles. The granules are arranged 
in lines which suggest the course of bacilli. It is generally 
impossible to distinguish individual bacilli by the above stains, 
though Loffler’s stain gives decided results ; but the parts which 
are thus doubtfully stained are those which with fuchsine show 
definite bacilli. If the section be rapidly stained by fuchsine, 
decolourised by acid alcohol, and counterstained by methylene 
blue, the cells are seen to be filled with a similar granular blue- 
stained material in which a few red-stained bacilli are embedded. 
It would, therefore, appear probable that the granular staining 
material is in large part made up of altered cell contents and 
possibly degenerated bacilli. 
It is impossible to distinguish with certainty these two 
bacilli by the use of various stains or by modifying the mate- 
rial in which they are dissolved, since the simple watery, the 
aniline oil, the alcoholic, and the carbolised solutions all stain. 
The rapidity with which the bacilli are stained by such a dye 
as fuchsine has been tried as a means of distinguishing between 
the two bacilli. The statements of different observers are very 
contradictory. 
Babes states that B. lepree is alone coloured by staining for 
thirty minutes with simple Poirier fuchsine and decolourising 
by an acid. Baumgarten gives two methods for staining the 
B. lepre and leaving the B. tuberculosis unstained. Ac- 
cording to this author, dilute alcoholic fuchsine (5—6 drops of 
the alcoholic stain in a small watch-glass of water) stains the 
B. lepre in twelve to fifteen minutes in sections, and on cover- 
glasses in five to six minutes, so as to resist decolorisation by 
acid alcohol (nitric acid 1 part, and alcohol 10 parts) for half 
a minute; or similar staining can also be effected in two to 
three minutes by Ehrlich’s fuchsine solution. The B. tuber- 
culosis is said to be left unstained. 
Bonomé, who was attempting to solve the question as to the 
nature of some lung lesions in leprosy, used Baumgarten’s 
