52 OQ. A. MAC MUNN. 
comes to the conclusion that Vogt and Yung! had not sufficient 
evidence for their conclusion that symbiotic alge are present. 
The sacculi which become stained red after the death of the 
animal were supposed by these observers to be symbiotic alge, 
and they held that the “‘ pyriform oil-cells ” of Wyville Thomson 
are the migrating amceboid spores of symbiotic alge. These 
were easily seen in the specimens examined by me, but they 
are totally different in shape and structure from any sym- 
biotic alge which I have seen. They appear to be out- 
growths from the surface of the pinnules, the flask-shaped ones 
being attached by the part representing the neck of the flask. 
Some which I measured varied in size from about 10, to5 mu 
in their long diameter, and from 6} to 54 in their short 
diameter. They are mostly homogeneous and of a yellowish- 
green colour ; some seemed to have granular contents, but these 
were of exceptional occurrence. 
I failed to find either starch or cellulose in these pyriform 
bodies. 
It was necessary not only to prove the presence or absence 
of chlorophyll, but also to determine whether Moseley’s ante- 
donin is present. 
Glycerine, water and alcohol readily extracted the red 
colouring matter, but ether failed to do so, as it only became 
faintly yellow. 
The glycerine extract was a fine rose colour. Spectro- 
scopically, a deep layer transmitted red and a little green, and 
in a shallow layer an ill-defined shading occupied the other 
half of green. Ammonium sulphide discharged the colour, 
leaving a faint yellowish tint, but no absorption bands were 
visible. Hydrochloric acid changed the colour to pale yellow, 
the solution showing no bands; and ammonia changed it to a 
faint rose tint. 
The aqueous solution gave the same kind of absorption as 
the glycerine solution, and showed in a thin layer only a faint 
shadow in the green. Ammonium sulphide discharged the 
colour of such a solution, leaving only a faint reddish yellow- 
1 «Traité d’Anatomie Comparée Pratique,’ libr. 7, 8, pp. 519—572, 
