CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 53 
tint, and no bands appeared: Hydrochloric acid, as well as 
acetic acid, also destroyed the colour of this solution. 
The absolute alcohol solution was of a fine rose colour ; a deep 
layer transmitted the red rays and a little of the green, but in 
the deepest layers there was not atrace of a chloro- 
phyll bandinthe red. A shallower depth showed an ill- 
defined shading in the green, best marked towards its blue end. 
Ammonia discharged most of the colour, leaving a faint reddish 
tint and some ill-defined shading at the blue end of green. 
Hydrochloric acid destroyed the red colour, changing it to 
yellow, and the solution now showed no bands. 
The ether solution did not show a trace of a chloro- 
phyll band in red; it was of a pale yellow tint, and in a thin 
layer a faint, ill-defined band was seen at the blue end of green. 
On evaporation it left a yellow residue which became a tran- 
sient dirty green with sulphuric acid, a distinct blue and green 
with nitric acid, and became more yellow with iodine in potassic- 
iodide. Therefore it would appear that the ether removed a 
yellow lipochrome, and left the rose-red pigment untouched. 
So far, then, one may conclude that the intrinsic colouring 
matter of Antedon rosacea is not identical with Moseley’s 
antedonin, which will be described for purposes of comparison 
further on, and that no chlorophyll is present. Therefore 
the absence of symbiotic alge may be safely inferred, 
I expected to meet with chlorophyll after a perusal of Kruken- 
berg’s paper! on the colouring matters of Antedon, entitled 
“Ueber die Farbstoffe von Comatula Mediterranea (Lam.), 
Antedon rosaceus (Frem.),”’ in which Krukenberg refers to a 
band in red evidently helonging to chlorophyll. It would 
appear, however, that he did not take the precaution of removing 
the contents of the stomach before putting the Antedon into 
solvents. The contents of the stomach when squeezed out on 
a glass slip and examined with the micro-spectroscope show 
the dominant chlorophyll band and others well marked, which 
are therefore due to the food. In my experiments I always 
removed the food from the stomach and washed the latter clean 
1 *Vergl. physiol, Studien,’ zweite Reihe, dritte Abth., 8. 88—91, 1882. 
