CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 61 
deep layer strongly absorbed the violet end of the spectrum, 
transmitting the red and a part of the green; in a thinner 
layer a band was seen from about 4503 to 468 in its 
darkest part, although it really began at about A526. This 
solution on evaporation left an orange residue soluble in ether 
with a yellow colour, and in chloroform with an orange colour ; 
the latter solution showed one band from about 523 to \ 471. 
The residue left by evaporating these solutions was coloured a 
transient blue and greenish by nitric acid, blue and green by 
sulphuric acid, and it was hardly changed by iodine in potassic- 
iodide, so that this rhodophan-like lipochrome differed in no 
essential respect from that of the blood. In the natural state 
the pigment was present as a lipochromogen, as it exists, for 
example, in the hypoderm of Crustaceans or in their exo- 
skeletons, to which I shall have to refer again. 
The respiratory tree showed no band. 
The ovaries were a fine, almost vermillion red; they trans- 
mitted the red and a part of green, and compressed into a 
thin layer a band was seen at the blue end of green. Extracted 
with absolute alcohol they yielded a salmon-coloured solution, 
transmitting in deep layer red and some green, and in a suitable 
depth showing a band shading more than half the blue end of 
green. Evaporated, this left a residue of ared and reddish 
yellow colour. ‘This residue was only partially soluble in ether, 
what remained after extraction being red. The ether extract was 
yellow, and gave a band at the blue end of green, its darker part 
from about A 507 to A 471, although the shading commenced at 
about A 520. On evaporation, the ether left a faintly yellow 
residue which became a transient blue with nitric acid, and 
greenish with sulphuric acid. 
The part insoluble in ether was soluble in chloroform, with 
a reddish colour, giving one band at blue end of green. This 
solution was remarkable for its pure red colour, and after 
evaporation it left a pale red residue which was more soluble 
in ether, with a yellow colour. ‘This residue became a transient 
blue and green with nitric acid, blue and greenish with sulphuric 
acid, and perhaps greenish with iodine in potassic-iodide. So 
