CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 67 
with nitric acid, green, also blue-green, and brownish, with 
sulphuric acid; and with iodine in iodide of potassium a 
yellowish green. Hence the integument contained at least one 
rhodophan-like lipochrome. 
The radial cca (so-called liver) when extracted with alcohol 
yielded a greenish-yellow solution with a red fluorescence, 
showing a well-marked enterochlorophyll' spectrum; in this 
solution only one lipochrome band evidently corresponding 
to that of the integument was seen. On evaporation and 
extraction with the lipochrome solvents, and testing the residue 
with the usual reagents, the lipochrome reactions were ob- 
tained. 
Krukenberg’ describes a pigment which he calls ‘‘ astero- 
cyanein” from Atropecten aurantiacus, soluble in water 
with deep blue violet colour; but its spectrum showed two 
bands, one between c and p, the other between p andr. He 
thinks it is related to “ cyanein” from Rhizostoma Cuvieri. 
At 80° C. it changed to wine red, and the bands disappeared. 
On cooling, a part of it became regenerated. It was unchanged 
by hydrochloric acid and ammonia. Alcohol, chloroform, 
and caustic soda acted on this pigment like heat. For its 
other characters the original may be consulted, but I may 
here say that the present violet pigment was not identical 
with ‘‘asterocyanein,” owing to the absence of absorption 
bands. 
Asterina gibbosa.—The ovaries were orange and showed 
a band at the blue end of green; extracted with alcohol they 
yielded a pale yellow solution which had a strong absorptive 
power for the violet end of the spectrum ; in a thinner layer a 
band was seen from about A 503 to A 474, and another in violet. 
The residue from this was a dull yellow colour ; it dissolved in 
chloroform with a yellow colour, giving a band at blue end of 
green, and another faint one in violet. It was soluble in 
ether with a paler colour, and in other lipochrome solvents. 
1 «Proc. Roy. Soc.,’ vol. xxxv, 1883, p. 370; and ‘ Philos. Trans.,’ pt. i, 
1886. 
2 «Vergl. physiol. Studien,’ zweite Reihe, dritte Abth., 1882. 
