CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 71 
red end of the spectrum. In a thinner layer still a little more 
of the violet came through than in the original rectified spirit 
solution. 
The rectified spirit extract became a greenish-brown with 
nitric acid, but the solution only absorbed the violet end of 
the spectrum, and showed no band. With still more nitric 
acid the solution seemed rather more green, but showed no 
bands. 
With hydrochloric acid the colour also became a dull brown- 
ish yellow, and showed slight turbidity. With caustic potash 
(as with ammonia) it became ruby red and showed a broad, 
ill-defined band at the beginning of green, and the violet was 
absorbed ; treated then with hydrochloric acid it became a 
dull yellow. On evaporation the rectified spirit solution left 
a dark brown, amorphous shiny residue. 
This residue was partially soluble in absolute alcohol, form- 
ing a yellow-brown solution, which absorbed the violet end and 
showed an ill-defined band at the beginning of the green. 
Ether took up some of this residue, which, after extraction 
with alcohol, appeared in parts brown and in parts green; the 
latter part seemed to go into the ether, which, however, was a 
dirty brown colour when looked at on a white dish. This solu- 
tion also absorbed the violet and showed the ill-defined band 
at beginning of green. 
The residue was also soluble in chloroform with a deep red- 
brown colour, and the solution had the same spectroscopic 
characters as the above. This chloroformic solution seemed 
dichroic, being brown in some lights, red in others. 
Bisulphide of carbon also dissolved it with a brown colour, and 
the solution showed the same spectroscopic characters. The 
pigment left by evaporation of an absolute alcohol solution 
seemed to be present under the microscope as very small greenish 
granules. This pigment was also soluble in benzene with a dull 
brownish-yellow colour, and, lastly, in water with a brown colour, 
also absorbing the violet end of the spectrum and showing no 
distinct band. On examining a living worm in the compres- 
sorium, under the microspectroscope, no band could be detected; 
