CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 73 
Hydrochloric acid changed the colour to a deeper blue, not red 
as in the case of bonellein ; but no new bands appeared. With 
an excess of acid it becamecloudy. I had not enough material 
to try the effect of alkalies, or to obtain other reactions. 
The solid pigment lost its green colour with strong nitric acid, 
and with strong sulphuric acid. Iodine in iodide of potassium 
produced no marked change. I hope to examine this pigment 
again more carefully. To prevent misconception I must here 
state that this pigment is not chlorophyll. 
The Chlorophyll of Chetopterus.—Professor Ray 
Lankester has shown that chlorophyll exists in this worm. I 
have not had an opportunity of examining a fresh specimen, but 
by the kindness of Mr. J. T. Cunningham I have been able to 
examine a spirit solution of the pigment. Owing to the 
worm having been killed in corrosive sublimate solution, and 
afterwards kept in methylated spirit, the chlorophyll had 
become slightly changed. This is well shown in sp. 10, the 
spectrum of the alcohol solution, which had a dark green 
colour and showed a fine red fluorescence. If we compare 
this spectrum with that of the modified chlorophyll figured by 
Sachsse,! we see that this solution contains “ modified ” chloro- 
phyll; and as Sachsse says, ‘‘ Band III des frischen Spectrum 
ist haufig ganzlich verschwunden oder hat, wenn sichtbar, 
nicht nur stark abgenommen, sonderen auch seine Lage 
geandert, indem es, im normalen Spectrum p nahe, jetzt 
naher an = geruckt ist. BandI... . ist kohlschwarz, auf der 
Seite gegen das rothe Ende ziemlich scharf abgeschnitten, auf 
der brechbareren Seite dagegen stirker schatten artig verlaufend 
als im Normalzustand.” ‘The bands of the alcohol solution of 
this chlorophyll read as follows:—Ilst began to be shaded at 
678, was black from 671 to 1658, and shaded off to 1641; 
2nd A619 to A589, 3rd a mere shading from about 569 
to A553°5, 4th A545 to X526, and 5th A514 to A490, 
The absence of distinct lipochrome (= xanthophyll) bands 
was noticeable ; and on subsequently agitating a spirit solution 
—sufficiently diluted with water—with bisulphide of carbon, 
* “Die Chemie und Physiologie der Farbstoffe, &c.,’ 1877, S. 47. 
