74 C. A. MAC MUNN. 
no separation into a green and a yellow solution could be 
effected. The bisulphide layer did come down green, but the 
upper spirit layer was also green and showed only the bands of 
the green constituent. This may have been due, however, to the 
disappearance of the lipochrome by the previous action of the 
perchloride of mercury and methylated spirit. 
A phyllocyanin-like spectrum was produced by the action of 
mineral acids on the alcohol solution ; thus hydrochloric acid 
produced the spectrum shown in sp. 11. It did not seem to 
alter the colour, as this remained as before. 
The following wave-length readings of the bands appearing 
in a solution treated by sulphuric and nitric acid respectively 
are only approximately true : 
H, SO, Bands. HNO, Bands. 
(1) a, 673 to 1626°5 (1) a, A673 to 1626°5 
(2) y, 4612 to X584'5 (2) y, 4612 to 584°5 
(3) 8, X577 to A550°5 (3) 6, A577 to X550'5 
(4) 8, 542 to A512 (4) 6B, 542 to A512 
(5) », 4507 to A478 (5) », 4507 to A478 
Acetic acid did not change the spectrum, as it already was 
that of modified chlorophyll. 
Ammonia caused the spirit solution to appear more yellow 
green, and appeared to narrow the 2nd and 8rd bands. 
Caustic potash had the same effect on the colour and spectrum, 
except that it made the 2nd and 3rd bands almost invisible ; 
the Ist band being also less shaded than before, and the 4th 
more distinct. 
Evidently Chetopterus insignis (Baird) contains a true 
chlorophyll, although Geddes! could not detect any evolution 
of oxygen on exposing Chetopterus Valenciennesii to 
sunlight ; but this is not to be wondered at, since the chloro- 
phyll is shut up within the animal’s body. 
Arenicola piscatorum? Besides the hemoglobin which 
—as Professor Lankester has shown—this worm contains, a lipo- 
chrome or lipochromes are also present. The black specimen 
examined yielded its black pigment to caustic potash, in 
1 Loe. cit., p. 379. 
