82 Cc. A. MAO MUNN. 
strongly. The band in red was about \ 680 to A 636'5, its darker 
part from 4 673 to 1654, <A second feeble chlorophyll-like 
band was also just visible. In a thin layer a band at the blue 
end of green extended from about A 500 to A 468 in its darker 
part. The ether solution left a chrome-yellow residue, soluble 
in chloroform, with a deep yellow colour showing also a chloro- 
phyll-like band in the red, and a second feeble one before p, 
with strong absorption of the blue end, and in a thin layer a 
broad band from about 1505 to 474 (=darker part). The 
brownish-yellow residue from this became a transient blue 
and greenish with nitric acid, dark brown, bluish, and violet- 
brown with sulphuric acid, and was unchanged by iodine in 
iodide of potassium. 
The part of the above residue insoluble in ether went into 
chloroform, but this solution appeared to be identical in every 
respect with the last-mentioned chloroform solution. Here, 
again, is a colouring matter which appears to be partially 
chlorophylloid in its nature, and yet is a true lipochrome. 
Krukenberg! has examined Botryllus though of another 
species. He says the Botryllus species at Trieste, when 
treated with lipochrome solvents, or with alkalies and sea- 
water, have their violet pigment changed into a yellow-brown 
colouring matter. The violet parts of the colony when touched 
with caustic potash become yellow-brown, but with acetic 
acid they again become violet. He says: “Es scheint aus 
diesen Befunden hervorzugehen, dass der violette Botryllus- 
farbstoff nur in festem Zustande bestehen kann, und dass 
seine Existenz an eine saure Beschaffenheit des gewebes 
gebunden ist.” Further, he states that its yellow-brown 
alcoholic solution (whose residue is soluble in carbon bisul- 
phide with an orange colour) has similar spectroscopic 
characters to that obtained from a black species of Didemnum, 
but differing from an orange species of the same. 
Botrylloides.—An orange-coloured specimen was extracted 
with absolute alcohol, to which it imparted a yellow colour with 
a faint tinge of red. This solution absorbed all the violet end 
1 *Vergl. physiol. Studien,’ zweite Reihe, dritte Abth., 1882, S. 106. 
