CONTRIBUTIONS TO ANIMAL CHROMATOLOGY. 83 
of the spectrum, transmitting red and a little green. In a thin 
layer it showed a band at the blue end of green beginning at 
about A520 and darker from 507 to A471. This left a 
residue partly yellow, partly reddish. A portion of this was 
soluble in ether and chloroform, and the remainder went into 
water; the ether being yellow and the chloroform more orange, 
and both showing one band in a shallow layer, in blue green. 
The residue from the ether solution was in parts reddish and 
in parts yellow green; the reddish part was not much changed 
by nitric acid, the greenish became perhaps slightly tinted 
violet. Sulphuric acid changed both parts of the residue a kind 
of orange; besides, in the greenish part a kind of violet tint was 
- noticed. Todine in iodide of potassium had no effect on either. 
The part of the residue soluble in water had a faint reddish 
tinge, but with the exception of a feeble shading at the blue 
end of green this solution gave no distinct bands. Although, 
therefore, closely resembling a lipochrome the orange colouring 
matter of Botrylloides failed to show the lipochrome re- 
actions. 
Amouroucium proliferum.—The orange colouring matter 
of this Ascidian goes into alcohol, so also does the bright red, 
both yielding an orange solution. In a deep layer this 
possessed a strong absorption for the violet end of the spec- 
trum, and showed a band in red closely resembling the prin- 
cipal band of chlorophyll. In a shallow layer one lipochrome 
band was visible, and perhaps a second in violet. The orange 
residue was soluble in ether, in chloroform, in bisulphide of 
carbon; in each case giving the same kind of spectrum as that 
seen in an alcohol solution. 
The residue became a distinct but transient blue, and dark 
bluish green with nitric acid, a green and blue with sulphuric 
acid, and was unchanged by iodine in iodide of potassium. So 
that here the colour was due to a red lipochrome. 
In Clavellina lepadiformis I noticed some bluish 
colouring matter which gave a feeble shading at p and some 
shading at the blue end of green. I could not change this 
bluish colouring matter into a lipochrome by alcohol, owing 
