CONTRIBUTIONS TO ANIMAL OHROMATOLOGY. 85 
largely increased, while the lipochromes come to occupy a very 
important part in their colouration, and as the results which 
have been described here show, it is getting more difficult to 
come upon new pigments. Most of those which I have lately 
met with, and which have recently been described by others, fall 
under classes of colouring matters which have already been 
described. 
Chrysaora hysocella.—Owing to the presence of Pro- 
fessor Moseley’s polyperythrin! in various Ceelenterates I was 
anxious to examine the brown colouring matter of jelly-fishes. 
In Chrysaora abundance of a brown pigment is present dis- 
posed in radiating triangular areas on the upper surface of the 
umbrella, and in dark patches, thirty-two in number, all round 
the margin of the disc, also in the tentacles; but in each of 
these situations it possesses the same properties. It also occurs 
dotted on the surface of the umbrella between the triangular 
pigmented areas. Microscopically, it occurs in granules, and 
is limited to the surface ; these granules are yellowish in colour 
under a high power. I attempted in vain to extract the 
brown colouring matter with alcohol, ether, chloroform, 
alcohol and sulphuric acid, alcohol and caustic potash. But 
when the portions of Chrysaora were allowed to stand, the 
sea-water contained in the tissues dissolved the pigment out 
readily, forming an orange-brown solution, showing a broad 
dark band at the blue end of green. When more pigment went 
into solution the fluid became a dark brown colour. Boiled in 
fresh- and sea-water the colour went into solution, but showed 
no bands except the shading at the blueend of green. A deep 
layer of this solution only transmitted red and some green. 
Ammonia and caustic potash precipitated the colouring matter. 
Hydrochloric acid did not discharge the colour at first, 
although it became much lghter; strong sulphuric acid and 
nitric acid discharged it after some time. Absolute alcohol 
also precipitated the pigment, the fluid becoming flocculent 
after some time. 
1 Quart. Journ. Micr. Sci.,’ vol. xvii, 1877, pp. 1—23. See also ‘Journ, 
Physiol.,’ vol. vii, No. 3, and vol. viii, No. 6, 
