64 EDWIN S. GOODRICH. 
lently to “boil up,” and hecomes dissolved, leaving only one 
(or two) minute yellowish and very refringent coagulated 
granules, which undergo no further change. Strong HCl and 
H,SO, have at first somewhat the same action as acetic acid, 
swelling up the thread to a transparent viscid mass, which, 
however, is not dissolved in the cold. Strong HNO, acts in 
the same way, the swollen thread being further reduced to a 
small granular mass, which, on heating, is broken up into 
globules, and finally dissolved on boiling (?). Osmic acid 
shrivels the thread a little, and stains it a pale greyish tint. 
With tannic acid it becomes reduced to an irregular shrivelled 
mass. The thread is insoluble in lime water,—in fact, the 
addition of lime water to the celomic fluid is one of the 
best methods .of showing up the threads in a mass of cells! 
(figs. 15, 16). 
The xanthoproteic test gives no result as with the white 
granules. The thread does not stain readily with eosin; but, 
on the other hand, it stains readily with methyl blue, and 
especially with methyl green. In an acidified solution of 
Victoria blue it stains dark purple, and again dark blue in an 
alcoholic solution of cyanin. 
The chief results are shown below in tabular form. The 
sign + means that the substance is soluble in the reagent, 
the sign — that it is not obviously soluble without heating. 
3 : | | | 
ducts in the celomic] :| S |x $4 sHESM Whe Re I Lol ie 5 
me meaertclee $3 3 a a3 z8 ac ea sig = = zd 3 
Ael| 4 ROB Re asel/Os|<4s/m m = Ssis 
Of Vermiculus— | | 
1. Large white | | 
granules . J + |+—/—| + |... | oe | + | + [+] + 4! + [+ 
2. Small residual | | 
granules . .| — | — |—| + | «| «. | + | + J+) + J+] = J= 
Of Enchytreus— | | | 
3. White granules) + | — /—| + | + | +/+] + /+! + [4/4—J— 
par- | 
tially, 
4. Thread. . J — | — |J—| —| - | —/] —] + ‘| a i a 
1 The thread does not give rise to double refraction. 
