392 LILY HUIE. 
of the cell, so as to give a fairly homogeneous appearance, 
large spherical cavities had appeared in the reticulum here and 
there, such cavities being occupied by the cell-sap. The sap 
had, moreover, a much darker pink tint. Thus a breaking 
down or destruction of some part of the reticulum has taken 
place. After some seventy-two hours’ stimulation this break- 
ing down of the reticulum had reached to such an extent that 
in the peripheral portion before referred to, all the central 
core of the meshwork had for the most part disappeared, and 
replacing it was a single large vacuole filled with cell-sap. 
The cytoplasm had, moreover, contracted from the upper or 
free surface of the cell-wall. In no case does the destruction 
and consequent vacuolation extend to the base of the cell, 
where the nucleus is situated. The nucleus is always sur- 
rounded by dense protoplasm; and there are grounds for 
believing that after very long stimulation, when all the secre- 
tion has been poured out, and before absorption begins, an 
active growth of the protopiasm takes place around the nucleus, 
and in the more basal portion of the cell.” 
«The view here taken (which is supported by certain of the 
staining reactions) with regard to secretion is that in the 
gland-cells the more peripheral network consists of protoplasm, 
together with a formed substance derived from it, and that the 
outpouring of the secretion is caused by the repeated breaking 
down (owing to stimulation) of the protoplasm into this formed 
substance, which is of a mucous nature, and which rapidly 
attracts water and escapes as the secretion to the external 
surface.” 
Gardiner does not mention what the staining reactions are. 
The paper I quote from is called a ‘‘ Preliminary” one. I be- 
lieve the author has never published a more complete account ; 
and, as far as I know, no one has with modern histological 
methods worked out the minute cytological changes which 
occur in the gland-cells, especially with regard to the nucleus 
and nucleolus and the staining reactions of the protoplasm. 
