402 LILY HUIE. 
nearest to the apex. They are remarkably plump, exhibiting 
a full and rounded contour. They stain of a purplish tint. 
Whether a definite nuclear membrane exists is doubtful, but 
in mid-focus the nucleus is sharply defined against the cyto- 
plasm, partly because of the difference in tint, and also because 
of the peripherally placed dark blue chromatin granules. 
The Nuclear Chromosomes are very minute granules, appa- 
rently shaped like diplococci, each coccus being slightly flat- 
tened, and attached to its neighbour by its broad side. They 
stain an intense blue without any reddish tint. 
The Nuclear Plasm is granular and always very dense in 
controls. Sometimes the granules appear to radiate from the 
nucleolus, and in other cases their arrangement produces a 
sponge-like appearance; or, again, the granules are too closely 
packed to show any definite arrangement. They stain of a 
purplish tint. 
The Nucleolus is embedded in the nuclear plasm. One, 
sometimes more, very distinct deep red nucleolus is always 
present, surrounded by a narrow Frommann’s clear zone. If 
there is only one nucleolus, it is large; it may be in any part 
of the interior of the nucleus, but is generally about one 
quarter to one-third of the diameter of the nucleus, removed 
from its periphery. 
It always presents a perfectly smooth spherical outline, and 
contains one or more distinct endonucleoli. 
Effects of Stimulation for One Minute on the 
Apical Gland-cells. Type 1. Fig. 2: 
The Cell Wall shows no evidence of diminished turgor, there 
being no collapse. It stains purplish. Between the apical 
thirds of neighbouring cells a very distinct reddish material is 
to be noted, staining, in colour and intensity, exactly like the 
semi-fluid egg-albumen in contact with the head. In tentacles 
stimulated with pieces of boiled cork this red material is 
always absent. We are dealing, therefore, with a substance 
(albumen ?) which is passing in between the cells, and not with 
a substance which is being excreted by the cell. 
