408 LILY HUIE. 
The Nucleolus is undiscernible. It is, however, probably 
present, as in other material, also, showing this retracted con- 
dition of the nuclear-plasm, I have been able to reveal it by 
Heidenhain’s iron-alum hematoxylin. 
Effect of Stimulation for One to Four Hours on the 
Apical Gland-cells. Fig. 8. 
The Cell Wall is very pale blue. 
The Cytoplasm is greatly vacuolated. The main body of 
the plasm is red, shading to blue round the vacuoles. 
The Nucleus is spherical and swollen. Its outline is very 
indistinct, and its periphery undefined. 
The Nuclear Chromosomes are deep blue. ‘They show a 
tendency to be displaced or arranged irregularly. 
The Nuclear Plasm is purple. It appears thin because spread 
out over a larger space than before. White spaces occur in it 
here and there. 
The Nucleolus is small and pale red. 
Effect of Stimulation for Twenty to Thirty Hours 
on the Apical Gland-cells. Figs. 9a and 10. 
Note.—The state about to be described is characteristic of 
glands that have been active for twenty to thirty hours, but is 
sometimes reached in twelve hours. 
The Cell Wall is pale blue. It presents the same appearance 
of loss of turgor as noticed in specimens fed for one to four 
hours. 
The Cytoplasm is an extremely scanty network staining red, 
or with a trace of blue here and there in the parts furthest 
from the nucleus. 
The Nucleus is normal as to size and outline, or is shrunken 
so as to form an oblong body with its long axis at right angles 
to that of the cell. 
Nuclear Chromosomes now consist of eight large dark blue 
segments. They are remarkably distinct and conspicuous in 
uushrunken nuclei, and appear to be V- or U-shaped, reminding 
one of the well-known stages in karyokinesis. In shrunken 
