AL 4 LILY HUi#. 
The Nuclear Plasm is less dense than formerly. Its colour 
is like that of the surrounding cytoplasm. 
The Nucleolus is unaltered from the last stage described. 
Effect of Stimulation for Twenty Minutes to One 
Hour on the Lateral Superficial Gland-cells. 
Fig. 18. 
Cell Wall is pale purplish blue. The walls of these cells do 
not show the same loss of turgor that is seen in the apical cells. 
Owing to their greater thickness they are more resistant. 
The Cytoplasm is reddish purple. It seems to cover as 
great an area as in control leaves, but is everywhere much 
less dense. There is no shrinkage from the cell wall. The 
peripheral layer is very blue. A blue corpuscle-like vacuole 
is sometimes present. 
The Nucleus is swollen, and its outline is very indistinct. 
The periphery is apparently broken, and there is no trace of a 
nuclear membrane. 
The Nuclear Chromosomes are in the form of a great number 
of irregular granules larger than in unfed specimens, but 
somewhat indistinct in outline, and staining a less intense 
blue. 
The Nuclear Plasm is less dense than formerly, because of 
the increased size of the nucleus. In it there are large white 
spaces. It stains a bluish purple, and so gives the nucleus a 
bluer tint than the surrounding cytoplasm. 
The Nucleolus is pale red. Frommann’s clear zone is not 
seen. The endonucleoli are not well defined, or are absent. 
Effect of Stimulation for One to Four Hours on the 
Lateral Superficial Gland-cell. Fig. 19. 
The Cell Wali is very pale blue. 
The Cytoplasm is red or reddish purple. 
The Nucleus may be said to be perfectly wanting in outline. 
It is chiefly defined from the cytoplasm by its distinct blue 
chromosomes upon their light background of sparse nuclear 
plasm, and large white spaces. 
