OHANGES IN CELL-ORGANS OF DROSERA ROTUNDIFOLIA. 417 
Tue Sreconp LAYER or GLAND-CELLS. 
The Resting State, seen in Unfed Leaves used as 
Controls. 
The Cell Wall is pale blue. 
The Cytoplasm is very scanty compared to cells of the 
outer layer. 
A thin peripheral plasmic layer always remains applied 
to the wall of the cell. Cytoplasmic strands suspend the 
nucleus in the middle of the cell, and radiate from it to the 
peripheral layer lining the wall. Large vacuoles occupy the 
spaces between these radiating strands. The structure and 
staining properties of the cytoplasm are exactly the same as in 
the apical and lateral gland-cells of the superficial layer. A 
reddish tint round the nucleus is sometimes (though rarely) 
seen. 
The Nucleus, Nuclear Chromosomes, Nuclear Plasm, and 
Nucleolus are like those of the lateral superficial gland-cells. 
The Effects of Stimulation for One Minute to 
Twenty Minutes upon the Gland-cells of the 
Second Layer. 
All the cytological changes correspond to those of the 
lateral superficial cells, except that the cell walls are always 
pale purple, never red. 
Effect of Stimulation for Twenty Minutes to One 
Hour on the Gland-cells of the Second Layer. 
The Cell Wall and Cytoplasm are like those of the superficial 
cells stimulated for a slightly longer period. 
The Nuclei of the cells below the apical gland-cells have 
shrunken in the same way as the apical nuclei, while the lateral 
cells of the second layer have their nuclei in the same con- 
dition as the lateral superficial cells, or only very slightly 
shrunken. 
The subsequent changes in the cells of the second glandular 
layer agree in all respects with those of the lateral superficial 
gland-cells. 
