﻿370 
  LEONARD 
  R0C4EBS. 
  

  

  Fig-. 
  8 
  of 
  liue 
  I 
  shows 
  a 
  nearly 
  full-sized 
  group. 
  All 
  stages 
  

   of 
  these 
  multiple 
  divisional 
  forms 
  occur 
  in 
  large 
  numbers 
  in 
  

   favourable 
  cultures 
  at 
  27° 
  C.^ 
  every 
  stage 
  being 
  sometimes 
  

   seen 
  in 
  a 
  single 
  field 
  of 
  the 
  microscope. 
  They 
  are 
  found 
  

   most 
  abundantly 
  in 
  a 
  slimy 
  material^ 
  which 
  appears 
  in 
  the 
  

   tubes 
  after 
  a 
  day 
  or 
  two^ 
  and 
  which 
  stains 
  rather 
  like 
  fibrin, 
  

   but 
  contains 
  very 
  few 
  red 
  corpuscles. 
  This 
  mode 
  of 
  division 
  

   also 
  takes 
  place 
  within 
  the 
  spleen 
  during 
  life, 
  probably 
  

   accounting 
  for 
  the 
  greater 
  number 
  of 
  the 
  parasites, 
  and 
  the 
  

   different 
  stages 
  can 
  be 
  seen 
  in 
  smears 
  made 
  from 
  the 
  organ 
  

   shortly 
  after 
  death. 
  The 
  smallest 
  multiple 
  form 
  is, 
  however, 
  

   very 
  rarely 
  seen 
  in 
  films 
  of 
  blood 
  obtained 
  by 
  spleen 
  puncture, 
  

   probably 
  because 
  the 
  cells, 
  distended 
  b}^ 
  a 
  number 
  of 
  the 
  larger 
  

   forms, 
  are 
  more 
  readily 
  ruptured 
  by 
  the 
  suction 
  action 
  of 
  the 
  

   syringe 
  than 
  are 
  those 
  containing 
  the 
  smaller 
  forms. 
  The 
  

   formation 
  of 
  these 
  multiple 
  young 
  forms 
  in 
  a 
  zoogloea-like 
  

   material 
  derived 
  apparently 
  from 
  the 
  protoplasm 
  of 
  the 
  

   dividing 
  parasite 
  itself, 
  and 
  occurring 
  in 
  culture-tubes 
  in 
  

   which 
  the 
  blood-corpuscles 
  have 
  broken 
  down, 
  clearly 
  proves 
  

   that 
  the 
  parasites 
  are 
  not 
  growing 
  in 
  the 
  red 
  corpuscles, 
  and 
  

   thus 
  renders 
  Laveran's 
  contention 
  that 
  tlie 
  parasites 
  are 
  

   piroplasma 
  untenable. 
  

  

  At 
  a 
  temperature 
  of 
  27° 
  C. 
  only 
  the 
  above-described 
  forms 
  

   were 
  seen 
  in 
  large 
  numbers. 
  Noye's 
  blood-agar 
  culture 
  

   medium 
  was 
  also 
  tried 
  without 
  success. 
  On 
  next 
  reducing 
  

   the 
  temperature 
  of 
  the 
  cold 
  incubator 
  down 
  to 
  about 
  22° 
  C 
  

   and 
  making 
  further 
  cultures 
  in 
  a 
  new 
  series 
  of 
  cases 
  of 
  

   citrated 
  spleen 
  blood, 
  further 
  and 
  more 
  important 
  changes 
  

   were 
  soon 
  found. 
  

  

  Developmental 
  Forms. 
  

  

  The 
  first 
  thing 
  noticed 
  was 
  an 
  enlargement 
  of 
  the 
  small 
  

   oval 
  spleen 
  parasites, 
  afi^ecting 
  especially 
  the 
  macro-nucleus 
  

   and 
  the 
  protoplasm 
  of 
  the 
  cell, 
  the 
  micro- 
  nucleus 
  remaining 
  

   unchanged. 
  Then 
  one 
  day 
  a 
  culture 
  of 
  only 
  twenty-four 
  

   hours' 
  growth, 
  the 
  fully 
  developed 
  flagellated 
  forms 
  shown 
  

  

  