﻿THE 
  DEVELOPMENT 
  OP 
  FLAGELLATED 
  ORGANISMS. 
  377 
  

  

  EXPLANATION 
  OF 
  PLATE 
  25, 
  

  

  Illustrating 
  Mr. 
  Leonard 
  Rogers' 
  paper 
  "On 
  the 
  Develop- 
  

   ment 
  of 
  Flagellated 
  Organisms 
  (Trypanosomes) 
  from 
  the 
  

   Spleen 
  Protozoic 
  Parasites 
  of 
  Cachexial 
  Fever 
  and 
  Kala- 
  

   Azar." 
  

  

  AH 
  the 
  drawings 
  in 
  this 
  plate 
  were 
  made 
  from 
  the 
  actual 
  specimens 
  by 
  the 
  

   Medical 
  College 
  artist, 
  Beliari 
  Lai 
  Das, 
  as 
  seen 
  under 
  a 
  y\j 
  lens 
  and 
  a 
  No. 
  4 
  

   ocular, 
  the 
  magnification 
  being 
  925 
  diameters. 
  The 
  preparations 
  were 
  all 
  

   stained 
  with 
  Romanosky's 
  stain, 
  used 
  by 
  Leishmau's 
  method. 
  

  

  Line 
  I, 
  Figures 
  1 
  to 
  4, 
  show 
  the 
  simple 
  method 
  of 
  division 
  of 
  the 
  spleen 
  

   parasites, 
  and 
  5 
  to 
  S 
  the 
  multiple 
  form 
  of 
  division. 
  

  

  Line 
  II 
  shows 
  the 
  organisms 
  present 
  in 
  a 
  film 
  made 
  from 
  freshly 
  obtained 
  

   spleen 
  blood, 
  Figure 
  8 
  representing 
  a 
  group 
  of 
  young 
  parasites. 
  

  

  Line 
  III 
  shows 
  the 
  forms 
  found 
  after 
  one 
  day's 
  incubation 
  of 
  the 
  same 
  

   blood, 
  the 
  parasites 
  showing 
  only 
  enlargement. 
  

  

  Line 
  IV 
  shows 
  the 
  same 
  after 
  two 
  days, 
  both 
  single 
  large 
  oval 
  forms 
  and 
  

   conjugating 
  ones 
  being 
  represented. 
  

  

  Line 
  V 
  shows 
  the 
  same 
  at 
  the 
  end 
  of 
  three 
  days, 
  both 
  conjugating 
  forms 
  

   and 
  elongated 
  pairs 
  being 
  present. 
  

  

  Lines 
  VI 
  to 
  IX 
  show 
  similar 
  development 
  day 
  by 
  day 
  of 
  Case 
  47, 
  the 
  early 
  

   flagellated 
  forms 
  being 
  seen 
  in 
  Figures 
  4 
  to 
  6 
  of 
  Line 
  IX. 
  

  

  Line 
  X 
  shows 
  the 
  large 
  flagellated 
  pairs, 
  with 
  the 
  flagella 
  arising 
  from 
  the 
  

   ends 
  containing 
  the 
  micronuclei. 
  

  

  Line 
  XI 
  shows 
  all 
  stages 
  of 
  the 
  development 
  from 
  a 
  one 
  day 
  culture 
  of 
  

   Case 
  37. 
  Figures 
  8 
  to 
  12 
  represent 
  the 
  fully 
  developed 
  long 
  trypanosome- 
  

   forms 
  with 
  macro- 
  and 
  micro-nucleus, 
  three 
  of 
  which 
  still 
  show 
  the 
  double 
  

   form 
  of 
  the 
  typical 
  development. 
  Figures 
  1, 
  5, 
  6, 
  and 
  7 
  show 
  the 
  small 
  

   flagellated 
  amoeboid 
  forms 
  resembling 
  those 
  found 
  by 
  Rose, 
  Bradford, 
  and 
  

   Plimmer 
  in 
  Trypanosoma 
  Brucii. 
  

  

  