﻿416 
  J. 
  W, 
  JKNKINSON. 
  

  

  or 
  nearly 
  so, 
  aud 
  do 
  not 
  differ 
  in 
  any 
  respect 
  from 
  those 
  just 
  

   described 
  except 
  that 
  the 
  outer 
  end 
  projects 
  slightly 
  from 
  

   the 
  surface 
  of 
  the 
  egg 
  (figs. 
  6 
  a 
  and 
  6 
  b). 
  The 
  chromosomes, 
  

   however, 
  are 
  beginning 
  to 
  diverge 
  by 
  their 
  apices, 
  and 
  we 
  

   can 
  see 
  in 
  many 
  — 
  though 
  not, 
  I 
  think, 
  in 
  every 
  case 
  — 
  that 
  

   these 
  divergent 
  points 
  are 
  still 
  connected 
  by 
  a 
  fine, 
  frequently 
  

   twisted 
  thread 
  (the 
  connecting 
  thread, 
  or 
  " 
  Verbindungs- 
  

   faden"). 
  Further, 
  the 
  pairs 
  of 
  chromosomes 
  are 
  not 
  jolaced 
  

   so 
  regularly 
  in 
  the 
  equatorial 
  plane, 
  but 
  many 
  are 
  scattered 
  

   over 
  the 
  spindle. 
  

  

  From 
  this 
  one 
  might 
  argue 
  that 
  we 
  are 
  dealing 
  here 
  with 
  

   a 
  late 
  prophase 
  of 
  mitosis, 
  and 
  this 
  opinion 
  is 
  certainly 
  

   strengthened 
  by 
  the 
  fact 
  that 
  the 
  ova 
  in 
  question 
  were 
  

   obtained 
  from 
  the 
  middle 
  part 
  of 
  an 
  oviduct 
  in 
  the 
  upper 
  

   portion 
  of 
  which 
  only 
  stages 
  of 
  the 
  first 
  polar 
  division 
  were 
  

   found. 
  On 
  the 
  other 
  hand, 
  the 
  commencing 
  divergence 
  of 
  

   the 
  chromosomes 
  aud 
  the 
  protrusion 
  of 
  the 
  outer 
  end 
  of 
  the 
  

   spindle 
  above 
  the 
  surface 
  of 
  the 
  egg 
  inclines 
  me 
  to 
  the 
  belief 
  

   — 
  though 
  I 
  cannot 
  express 
  a 
  very 
  positive 
  opinion 
  — 
  that 
  these 
  

   spindles 
  are 
  in 
  reality 
  in 
  the 
  condition 
  of 
  the 
  early 
  anaphase. 
  

   As 
  a 
  possible 
  explanation 
  of 
  the 
  irregular 
  position 
  of 
  the 
  

   chromosomes 
  in 
  the 
  spindle, 
  I 
  may 
  add 
  that 
  it 
  is 
  not 
  unknown 
  

   — 
  a 
  case 
  is 
  described 
  by 
  Boveri 
  (1888), 
  for 
  example, 
  in 
  the 
  

   egg 
  of 
  Ascaris 
  — 
  for 
  both 
  chromosomes 
  of 
  a 
  pair 
  to 
  pass 
  to 
  one 
  

   pole. 
  

  

  (c) 
  Anaphase 
  (figs. 
  7 
  a 
  and 
  7 
  h). 
  — 
  In 
  the 
  later 
  anaphase 
  

   the 
  daughter 
  chromosomes 
  pass 
  in 
  the 
  ordinary 
  way 
  to 
  the 
  

   opposite 
  poles, 
  where 
  their 
  apices 
  converge. 
  Between 
  them 
  

   the 
  general 
  fibres 
  of 
  the 
  spindle 
  are 
  clearly 
  apparent 
  ; 
  the 
  

   fibre-bundles 
  to 
  which 
  the 
  chromosomes 
  were 
  attached 
  can, 
  

   however, 
  no 
  longer 
  be 
  distinguished. 
  The 
  external 
  fibres 
  

   have 
  the 
  same 
  relations 
  as 
  in 
  the 
  previous 
  stage. 
  

  

  The 
  outer 
  pole 
  of 
  the 
  spindle 
  is 
  occupied 
  by 
  a 
  dense 
  hyaline 
  

   mass, 
  which 
  passes 
  together 
  with 
  some 
  of 
  the 
  superficial 
  

   pigment 
  of 
  the 
  egg 
  into 
  the 
  small 
  projecting 
  disc 
  which 
  

   marks 
  the 
  first 
  appearance 
  of 
  the 
  second 
  polar 
  body. 
  

  

  The 
  second 
  polar 
  body, 
  when 
  fully 
  formed 
  (fig. 
  8), 
  is 
  a 
  

  

  