184 JOHN E. S. MOORE. 



those appearances apparently indicative of such structures 

 were absolutely illusory. 



The definite nature of the whole archoplasmic structure, 

 however, in this undifi'erentiated tissue will, on comparison 

 with that figured and described by Hermann and Meves,^ be 

 obvious at once. 



Whether this diflPerence finds an explanation in the much 

 younger condition of my material^ or in some happy mani- 

 pulative chance 1 can ofi'er no direct opinion, but incline 

 rather to the belief that all those cells figured by Hermann 

 were taken during a phase of structural metamorphosis when 

 the body, though extremely large, is most difficult to dif- 

 ferentiate as a concrete whole, growth being always inversely 

 proportional to distinctness, as in figs. 13 — 16, 2, 3, 4_, 

 11, 10. 



Notwithstanding this it is possible, however, by appropriate 

 preservation and staining (see below) , to determine that all the 

 resting cells of the genital ridge present distinctly bounded 

 archoplasmic figures — often, it is true, ovoid (fig. 7), elongated 

 (fig. 9), or bent like a German sausage round the nuclei, to 

 which they may or may not be closely applied.^ 



The extreme pallor of the archoplasm when large and ex- 

 panded, as it is towards the advent of mitosis, calls for special 

 manipulation, as I have just stated. I have been most suc- 

 cessful in the use of a combination of Flemming^s triple stain 

 with the reduction process of Hermann, or by staining with 

 hsematoxylin after such reduction. 



The sections were stained very black, and washed out with 

 acid alcohol. 



When we have become assured of the presence of an archo- 

 plasmic body, it is often possible, in this condition of the 



• 'Anat. Anzeiger,' 1891, vi, " Ucber amitotische Kcrnteilung iu den 

 Spermatogouien des Salamanders uud Vcrhalten der Attraktionsspbiire bei 

 derselben. 



2 Herniann's results were obtained from spermatocytes of salamander at 

 the end of the summer. 



^ Cf. Meves' description of the " Spermatogouien," loc. cit. 



