ARCHOPLASM DURING MITOSIS IN LARVAL SALAMANDER. 185 



cell, to discern its contour when the tissue has been treated 

 by Hermann's method alone. 



By whatever means it has been brought into view, there is 

 always presented in this particular phase of cell life one or 

 two central bodies (figs. 3, 4), immediately surrounded by the 

 light zone, "medullary corpuscle" of van Beneden (figs. 

 3, b, 4), across which can be traced a few broad radial bands, 

 putting the central body (or bodies) in connection with a large 

 and pale granuious sphere, the archoplasm (fig. 3, c), the radia- 

 tion of whose granules is centred to the ''medullary zone" as 

 a whole, and not directly towards the central body, as in the 

 " spheres " of van Beneden. 



The further relation of these constituent parts and the 

 general protoplasmic radiation of the cell are identical with 

 that obtaining in the segmentation spheres of Ascaris. 



As I have already said, all these parts are never visible at 

 once in any attraction- sphere of Flemming; but it may be 

 borne in mind, from what has gone before, that the archoplasm, 

 prior to the onset of mitosis, is extremely difiicult of diflferen- 

 tiation. 



Further, in this condition the central bodies and their imme- 

 diate zone (medullary corpuscle) are far more easy of diagnosis 

 than the archoplasm is itself, as in fig. 2. 



And it is no less certain that this condition of things is 

 presented by far the greater number of the cells in the genital 

 ridge (of the salamander), and I believe elsewhere, owing to 

 its long duration. 



All these considerations may perhaps, to some extent, 

 account for the conspicuous absence of description of any- 

 thing clearly comparable to the archoplasm (outer portion of 

 the "sphere-attractive"), or the ''Nebenkern" in any of 

 Flemming's magnificent researches. 



There is, however, in this author's second paper in the 

 thirty-seventh volume of the ' Archiv ' (his figs. 25 and 26) a 

 delineation at least very suggestive of an archoplasmic body, 

 and in the latter figure it occupies a very remarkable position, 

 as the sequel will show. 



