AROHOPLASM DURING MITOSIS IN LARVAL SALAMANDER. 187 



I trusty then, that in the above I have made it clear how 

 certain objections to a complete structural homology between 

 the " sphere-attractive " of vau Beueden and the archoplasmic 

 bodies in the spermatocytes of Helix and Amphibia, are 

 removed by the observations I have recorded in the undiffer- 

 entiated genital ridge of the salamander ; and how the dif- 

 ference existing between the spheres described in these, and 

 those from various types of cells by Flemming, may in part 

 result from the archoplasm being difficult to put " en evi- 

 dence/' in the prevailing condition of the resting cells. I 

 now pass on to a confirmation and extension of the above 

 results, obtained by the discovery of bodies strictly comparable 

 to the archoplasm (" Nebenkern ") in the leucocytes of the 

 same larval salamander. 



So far as I am aware no such bodies have ever been 

 described in any leucocytes before. 



The able researches of Flemming on this subject^ show the 

 relatively large central body (always single in his figures) as 

 related to a marked and simple radiation, extending through 

 but a fraction of the granular cell-mass, the archoplasm as 

 well as the " medullary corpuscle " of van Beneden being 

 entirely absent. 



In fig. 23 I have copied two of Flemming's leucocytes, in 

 order to facilitate a comparison between the spheres repre- 

 sented there, and those of my figs. 1 7 — 19. The remarks I have 

 already made respecting the extreme difficulty with which the 

 archoplasm is differentiated during certain phases of its life 

 apply here as elsewhere, only more so ; for the dark nature of 

 these elements renders the coloration obtained from a com- 

 bination of Flemming's and Hermann's methods (p. 184) only 

 successful under exceptional circumstances, and then only 

 by dint of an objectionably bright light and small diaphragm. 



The use of hsematoxylin is preferable to the triple orange 

 stain, but I have found the simple reduction process of Her- 

 mann peculiarly adapted to this portion of the work. 



In leucocytes thus treated there appears a dense spherical 

 > ' Archiv fiir mikroskopische Anatomic,' Bd. xxxvii, pp. 249 — 297. 



