THE COLLAR-CELLS OF HETEROCGILA. 39 
the tedious care used for the tissue in mass. I believe the 
form of the cells in Vosmaer’s drawings has been influenced 
by this process, though the oval outlines of nuclei and vacuoles 
in the sections is probably attributable to the razor. Passage 
into glycerine is of course attended with the same necessity 
of preliminary passage into alcohol, but comparison with 
similar sections stained on the slide and mounted in Canada 
balsam show that the cells in glycerine are only equally dis- 
torted or coutracted, and, as these authors state, the collars 
and flagella are more visible, and the preparation very brilliant. 
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- Metscunixorr, E.—1879, ibid., vol. xxxii, p. 349. 
. Herer, K.—1886, ‘ Arb. Zool. Instit. Wien,’ vol. vi, p. 175. 
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10, LENDENFELD, R.—1889, ‘ Zool. Anz.,’ xii, p. 361. 
11. LENDENFELD, R.—1889, ‘ Zeitschr. wiss. Zool.,’ vol. xlviii, p. 406. 
12. Denpy, A.—1890, ‘ Quart. Journ. Micr. Sci.,’ vol. xxxii, p. 1. 
13. Cuatin, J.—1890, ‘Comptes Rendus,’ p. 889. 
14. Denpy, A.—1891, “A Monograph of the Victorian Sponges,” part 1, 
Melbourne, ‘ Trans. Roy. Soc. Vict.,’ vol. iii, p. 1. 
15. BippER, G.—1891], ‘ Quart. Journ. Micr. Sci.,’ vol. xxxii, p. 625. 
16. Mincuin, E. A.—1892, ibid., vol. xxxiii, p. 251. 
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San oar dOndD & 
