274 A. B. MACALLUM. 
Fig. 45, but also stained with eosin, which deeply colours the nucleoli and 
non-iron-containing constituents (see text). x 1640. 
Fie. 48.—Nuclei of the epithelial cells of the intestinal mucosa of Nec- 
turus lateralis. Alcohol, sulphuric acid alcohol thirty-six hours, acid ferro- 
cyanide mixture, balsam. The preparation from which 4 was drawn was, before 
being mounted in balsam, stained with safranin. x 1240. 
Figs. 49a and 4.—Two examples of Euglena viridis. Alcohol, sul- 
phuric acid alcohol thirty-six hours, acid ferrocyanide mixture, balsam, 
x 820. 
Fie, 50.—A portion of a nuclear filament from the “salivary” gland of a 
larva of Chironomus. Alcohol, sulphuric acid alcohol thirty-six hours, 
acid ferrocyanide mixture, balsam. x 1640. 
Fics. 51a, 6, ce, d.—Cells and portions of filaments of Oscillaria 
Froelichii; 4 and d represent the isolated cells as seen through their trans- 
verse walls. The “‘cyanophycin”’ granules are coloured red. Alcohol, sul- 
phuric acid alcohol three hours, acid ferrocyanide mixture, picro-carmine, 
balsam. xX 1640. 
Fie. 52.—A portion of a filament of Microcoleus terrestris. Alcohol, 
sulphuric acid alcohol three hours, acid ferrocyanide mixture, balsam. x 
1500. 
Fic. 53.—Examples of an organism obtained from sewage water (see text). 
Alcohol, sulphuric acid alcohol eight hours, acid ferrocyanide mixture, balsam. 
x 2000. 
