JOURNAL OF ENTOMOLOGY AND ZOOLOGY 131 



KOH, 10% and stronger, used after fixation with heat. In some 

 a fine reticulum of fibers or fibrils was shown with a pinkish stain; 

 the cells were not evident. 



KCN, saturated solution in water. Direct action on fresh tissues, 

 no detail in cells, fibres pink. 



Potassium Permanganate after fixation with heat, 5% solution. 

 All deep blue, deep colored fibers, cells not evident, coarse reticu- 

 lum between spaces. 



"Osmic acid," 1% after heat fixation, fibres straight, reticulum 

 only at edges. Center of ganglion dense mass of fibers and fibrils. 

 Cells deeply colored. 



The same reagent used as a fixer gave the same general appear- 

 ance, only more distinct, with less reticulum. Massed fibers in 

 places not separated into fibrils. 



Phenol, about 10% after fixation with hot water. Pink fibrils, 

 blue centers of cells, difference between cells and fibers not marked. 

 Possibly cell membrane is broken down, as the cells seem continuous 

 at all points with surrounding fibrils. There are a few vesicles in 

 the cells. Nuclei are deep blue, slightly granular or vesicular. 

 Direct action of the reagent gives similar results with more detail 

 in the nucleus; often there is a blue spot in the center of the nucleus 

 and an irregular cell body with pink fibrils that resemble a reticulum 

 in places. 



Chloroform, used after fixation. Strands of fibrils are clearly 

 marked, cells deep blue, pink at the edges of the cells. 



Chloral hydrate, about 20% aq. sol. after fixation. Cells less blue 

 or pink fibrils. No detail in the cells. 



Nitrate of silver before and after fixation with hot water. No 

 details in cells; coarse reticulum in places. 



Potash alum, saturated aq. sol. After fixation fibers and fibrils 

 pink, cells blue centers, pink margins; no details. 



Strychnine after fixation, saturated solution. Fibril reticulum 

 a dull pink, open net work not clearly marked. Cells uniform blue, 

 nuclei clear. In an older specimen more detailed reticulum, cells 

 all dark. In this there is a reticulum with large and small holes. 



