PHARYNGEAL GLAND OF THE EARTHWORM 43 
basophile protoplasm does not show any special structure, and 
it appears to contain a diffused chromatic substance (extra- 
nuclear chromatin). In sections of the glandular cells of Lu m- 
bricus sp. prepared by Champy’s method (fixation in 
Champy, postchromization followed by Iron Haematoxylin) 
the protoplasm is seen to contain a number of bodies which 
are probably mitochondria (T'ext-fig. 8). These protoplasmic 
bodies appear as irregular, curved and ramified filaments or 
TEXT-FIG. 3. 
Glandular or salivary cell of Lum bricus sp. showing a vesicular 
nucleus with large nucleolus and with numerous intraproto- 
plasmic mitochondrial bodies, x 2,200. 
patches composed of small darkly-stainmg granules, and are 
distributed throughout the protoplasm, not bemg confined to 
its basophile portions. Their number and size varies in different 
cells, some of which are crowded with them, while in others they 
are more or less scattered. 
As to the nature of the granular substance fillmg the clear 
parts of the protoplasm of these cells, from the sections pre- 
pared by an ordinary method (fixation in Bouin and staiming in 
Haemalum), I had already ample evidence that it is ordinary 
mucin. On the other hand, as the supposition of a secretion of 
mucin by these cells was absolutely denied by Stephenson, I had 
to study these glands in sections prepared by special methods 
