Q4 Ww. N. F. WOODLAND 
the result I obtained after making a wound on the ventral 
surface of an original tail. In this case the tail evidently 
autotomized at the autotomy plane separating the two segments 
involved in the wound, and the surface thereby exposed 
produced two tails. The upper tail was a normal regenerated 
tail in every respect, but the larger lower accessory tail differed 
in the essential respect that it was entirely devoid of a carti- 
laginous tube (Text-fig. 5, M”). 
Text-fig. 5, N shows another small accessory tail produced 
as the result of a wound on the ventral surface of a regenerated 
tail. An endoskeleton was also absent in this case, as also in 
another similar case which I have not recorded. 
In Text-fig. 5, E’’’ is shown a small accessory tail produced 
as the result of the oblique dorso-ventral cut already described 
(Text-fig. 5, E). The lower lobe of the bifid tail was devoid 
of a cartilaginous tube. 
I have described these four examples of accessory tails 
because, to judge from the paper by Tornier, the reader might 
imagine that an accessory tail without a cartilaginous tube is 
an impossibility. This is by no means the case, as these four 
examples and the examples in Anolis grahami, described 
by Brindley in 1898, prove. Assuming the statements of 
Tornier to be correct, it would appear that the injury must 
reach the vertebral column in order that the accessory tail 
produced may contain a cartilaginous tube. 
Novrrs on ‘TECHNIQUE. 
All Geckos were kept in large flower-pots, covered over with 
mosquito-netting, and were fed on house-flies. Tails preserved 
for section-cutting were fixed for 24 hours or longer in a saturated 
solution of potassium bichromate (100 parts), to which 5 parts 
of acetic acid had been added ; they were afterwards washed 
in running water for the same length of time, and then kept in 
70 per cent. alcohol until required for use. For the study of 
the gross structure of the tail, nothing is better than thick 
hand-sections (longitudmal and transverse) of the spirit- 
preserved material, dehydrated and mounted unstained in 
