GONOSPORA MINCHINII 159 
certainly lies as a rule outside the egg-cell, between it and the 
membranes (figs. 2, 4). It compresses the egg more and more 
ag it grows and is separated from it by a space, except at that 
one region opposite the point of entrance where the epimerite 
of the parasite adheres closely to the egg-cytoplasm near the 
germinal vesicle (fig. 9). Here are developed, in that part of the 
gregarine which is fixed to its host, small club-shaped bodies 
staining deeply in haematoxylin or fuchsin. They appear to be 
hollow, with long narrow necks reaching to the surface (fig. 9). 
These strange structures somewhat resemble the * lamelles 
mucoides ’ described by Léger and Duboseq in Nina (2); but 
their function would appear to be connected with the absorption 
of nutriment from the egg, or possibly merely with fixation. 
Meanwhile, as the parasite grows it enlarges the deep depres- 
sion it causes in the egg; the margin of this hollow is at first 
smooth (fig. 2), it soon becomes notched, and finally drawn out 
into delicate protoplasmic processes converging towards the 
‘point of entrance (figs. 8, 4, 5). 
Eifect of parasite on host egg.—tThe very young 
ovum has little or no yolk ; but with advancing age the yolk 
eranules increase in number until the fully-developed egg 
becomes so heavily loaded that it looks quite opaque. In 
parasitized eggs, however, the yolk is absorbed by the sregarine 
almost as fast as it is laid down, so that in late stages the com- 
pressed ovum is relatively clear, while the parasite on the 
contrary is densely granular (fig. 4). The nucleus of the egg 
is also influenced, for its nucleolus, instead of undergoing the 
orderly series of changes seen to occur in normal eggs, lags 
behind in differentiation, remaining in fact apparently at that 
stage of development it had reached when the egg was invaded. 
Thus the nucleolus in most parasitized ova resembles that of the 
quite young ovum when it is still small and has but little yolk 
(figs. 2, 7). 
Another peculiar and somewhat similar effect is seen on the 
egg-envelopes. There is no reason to think that the peri- 
vitelline layer when once formed can be reabsorbed, and since 
it is, as a rule, almost or quite absent from parasitized eggs, 
