176 HIROSHI OHSHIMA 
of caleareous deposits in Holothuria vagabunda, read 
on February 21, 1903. Here he spoke again on the sequence of 
the appearance of the secondary tentacles. Judging from the 
figure he indicated a difference from his former statement, since 
the dorsal tentacle (‘Text-fig. 1, II,) was shown as the first to 
appear among the secondaries. 
Having been engaged in the study of holothurians since the 
lamentable death of Professor Mitsukuri on September 17, 
1909, I have made a new and careful examination of the subject. 
On August 12, 1910, my first attempt ended in failure because 
the spawning season was already over in that year. During 
TExt-Fics. 2 & 3. 
a | 
5] ZAN| 2 § ~~ 
& 
2 , : 
/ \ 3 Bil 7~ 
A 1 4 i 3 4 5 
— tj S a 
= oe SE 
1 SON 
| : 1 Aelia 
Diagram showing the manner of Diagram showing the order of 
branching in tentacles of the appearance of pedicels in the 
young, A=NSide view of a part young. Ventral view. Numbers 
of the stem to show the spiral 1-8 indicate the order of appear- 
arrangement of ‘ pinnules’ 1, 2, ance, (After Mitsukuri.) 
3. B= Profile of the stem to show 
positions of pinnules. (After 
Mitsukuri.) 
the next few years I was unable to visit Misaki at the right 
season, but at last, on July 25 and Aucust 1, 1916, I was fortunate 
enough to observe the animal spawn and to rear the larvae. 
By the kindness of Professor Dr. 1. [jima I was permitted to 
-examine the valuable material left by the late professor, on 
which, and on my own collection, my studies have been based ; 
and incomplete though it may be, owing to several gaps in the 
developmental stages in the materials available for examination, 
