CYTOPLASMIC INCLUSIONS OF THE GERM-CELLS 269 
cobalt-nitrate-silver method (8), the magnification 1s too low 
to show the minute structure of the bead ; at Nn is the nucleus 
of the cells of the epididymis wall; and at Ga the Golgi 
apparatus of these cells. In all preparations we possess, the 
Golgi apparatus of the epididymis wall and of the bead- 
contents of the middle-piece are the only objects which go 
black with the reduced silver. In Pl. 11, fig. 3, a nearly ripe 
Cavia spermatozoon is drawn to illustrate the more minute 
structure of the bead (4ax) after treatment with Cajal’s method. 
The question now arises: What relation does the impreg- 
nating middle-piece bead (Gax in Pl. 11, fig. 8) bear to the 
Golgi apparatus of the spermatid cell (Ga in Pl. 11, fig. 8, and 
en in Pl. 12, figs. 7, d&c.) ? 
Extensive trials were made with Golgi apparatus techniques, 
and our best preparations were examined independently by 
both of us. We believe that the conclusion which each of us 
has arrived at independently is the correct one, but at the same 
time it is recognized that to come to a definite conclusion is 
difficult. 
In PI. 11, fig. 2, is drawn a ripening spermatid in which the 
Golgi apparatus (GA) lies in the hinder part of the cell. It is 
from a preparation made by Cajal’s unmodified Golgi apparatus 
method, and the mitochondria appeared as leht golden 
spheres (m). The most striking point to be noted is the 
undoubted double structure of the Golgi apparatus, which has 
a distinct bead projecting from its surface on one side (GAx). 
At this stage in the development it 1s possible to find pockets 
of cells within the testis in which every Golgi apparatus has 
this double appearance. If the spermatid be examined at 
earlier stages such as in Pl. 11, fig. 18, the bead (@ax) can still 
be seen as a swelling on the surface of the Golgi apparatus. 
With Cajal, Da Fano, or Kopsch methods, it is found that 
this outgrowing bead is not homogeneous—its centre being 
formed of a more lightly impregnating material, closely 
resembling archoplasm in its appearance. If, moreover, ripe 
spermatozoa are fixed in some such mixture as Flemming or 
Hermann, and stained in acid fuchsin, it will be noted that the 
