CYTOPLASMIC INCLUSIONS OF THE GERM-CELLS 989 
14. DESCRIPTION OF PLATES 11 anp 19. 
Mlustrating Dr. J. Bronté Gatenby and Mr. J. H. Woodger’s 
paper on the ‘ Cavy Sperm ’. 
Explanation of Lettering. 
A=acrosome. APG =proacrosomic granules. AR =archoplasm (centro- 
sphere). c, cl, c? =centrosome (first and second). ca =covering membrane 
of acrosome. cH=chromosome. cHB =chromatoid body. Ga =Golgi 
apparatus plus archoplasm. Gax =middle-piece Golgi apparatus elements. 
GAXxc =cytoplasmic bead containing the Golgi elements of the middle- 
piece. GE =Golgi element or dictyosome. 1Z4 =inner region of acrosome, 
Lev =lower part of archoplasmic vacuole embracing nucleus. mM =mito- 
chondrium, Mc =manchette. mp =middle-piece. mx =degenerate mito- 
chondria coalescing to form von Ebner’s granules. N=nucleus. oza = 
outer zone of acrosome. T =tail of sperm. v=archoplasmic vacuole, 
v.v. =archoplasmic vacuoles. 
In each plate the scale is on the left-hand side, 
Puate 11. 
Fig. 1.—Spermatid of Cavia, at the time when the Golgi apparatus 
(GE and 4R) is still in contact with the forming acrosome: the latter 
is distinguishable into two regions, an inner zone applied to the nucleus 
(1Z4) and an outer zone (024). The mitochondria are scattered throughout 
the ground cytoplasm. This cell is drawn froma Mann-Kopsch preparation ; 
note the discrete dictosomes, GE. 
Fig. 2.—Later spermatid, showing the double appearance of the Golgi 
apparatus (GA). At GAx is the bead which later becomes attached to the 
middle-piece, as in fig. 3 at Gax. Preparation by Cajal formalin-silyer 
nitrate method. 
Fig. 3—Ripe sperm just before the residue cytoplasmic bead strips off. 
The middle-piece Golgi apparatus bead is at GAX, the definitive Golgi 
apparatus, which is cast off, at Ga. Preparation by Cajal’s method. 
Fig. 4.—Sperm at same stage showing mitochondria heavil y impregnated 
by reduced OsO,. Preparation by Mann-Kopsch method. The ¢ r0lgi 
apparatus did not impregnate in the region of the testis from which this 
cell was drawn, The bead protoplasm is seen at Gaxc. 
PLATE 12. 
This plate is drawn from three separate sets of preparations by (a) Mann- 
Kopsch, (6) Cajal’s Golgi apparatus technique, (c) a mitochondrial method. 
