OVARY AND OVARIAN EGG OF ANOPHELES 415 
cut into a number of sections and the idea of the continuity 
of the convoluted bands is lost. 
Towards the centre of the nucleus a large irregular nucleolus 
is found imbedded in a mass of linin. This is joined to the con- 
voluted bands by linin threads. The nuclear membrane is 
very thick and is always plainly visible. 
When the follicle has reached the resting stage each nurse- 
cell nucleus is found to be surrounded by a large mass of 
cytoplasm which is limited by a definite cell-membrane. The 
cytoplasm is slightly granular and takes up rather more stain 
than that of the younger cells. 
The earliest stages of the oocyte nucleus which I have been 
able to distinguish contain a number of deeply-staining 
chromatin loops all of which arise together from one side of 
the nucleus (fig. 12). This is evidently the ‘ bouquet stage ° 
of the prophases. Usually a small nucleolus is also seen, and 
in the few cases I have observed in which this is not visible it 
is possible that it was hidden by the chromatin threads. At 
this early stage the nucleolus is a spherical vesicle and only 
stains very lightly. 
As the nucleus grows the nucleolus becomes relatively larger, : 
takes up stain rather more readily, and soon several vacuoles 
become visible in it. While this is taking place the chromatin 
threads wind themselves round the nucleolus and invest it 
tightly (fig. 13). The nucleolus continues to grow at a more 
rapid rate than the rest of the nucleus, while the chromatin 
strands do not appear to grow at all. The result is that the 
enlarging nucleolus gradually pushes the investing chromatin 
strands off itself, and these are then seen as a small mass of 
closely-woven threads at one side of the nucleolus (fig. 16). 
These threads concentrate into a closely-packed mass in which 
the individual chromatin threads can no longer be distinguished, 
and the whole has the appearance of a small dark nucleolus at 
the side of the true nucleolus. For want of a better term 
I shall refer to this mass as the ‘ chromatin residue’. This is 
embedded in a mass of linin which also invests the nucleolus and 
thus holds the two closely together (fig. 17). This arrangement 
