Review 
The Microtomist’s Vade-Mecum, by Arruur Boies Les. 
Sth edition, edited by Professor J. Bronr& GaTENBY. 
London, J. A. Churchill, 1921. Price 28s. net. 
Tuts eighth edition of Mr. Bolles Lee’s well-known Mzero- 
tomist’s Vade-Mecum has been edited and entirely revised 
by Professer J. Bronté Gatenby with the assistance of five 
collaborators. 
Readers of this Journal will not be surprised to find that 
Professor Gatenby has himself written special sections dealing 
with chromatin, chromosomes, and cytoplasmic inclusions in 
which he gives us the full benefit of his thorough practical 
experience. Moreover, he has re-written the parton Mammalian 
Embryological methods. An innovation is the inclusion of two 
new methods for staining bacteria in tissues which will doubtless 
be very useful to biologists not versed in_ bacteriological 
technique. In the next edition we may hope to see mentioned 
the important ‘ Carmine Claudius’ method for differentiating 
yeasts, as well as Gram-positive bacteria in tissues. 
Professor Gatenby is also personally responsible for a very 
valuable chapter on the cultivation of tissues ‘in vitro’. 
Zoologists will be grateful to Professor Bayliss for re-writing 
the chapter on Staining. Here we find an authoritative 
general account of the principles involved in the staining 
of living as well as dead cells. A careful reading of this lucid 
summary will save histologists from many pitfalls. 
The chapters on Neurological Techniques have been to a great 
extent re-written by Dr. Da Fano. The additional directions 
given for the carrying out of the Bielschowsky and other 
complicated impregnation methods will be much appreciated. 
For an important section embodying some of the new work 
in micro-chemistry on the lipoids and true fats and their 
differentiation we are indebted to Dr. W. Cramer, while 
Mr. J. T. Carter has revised the section on bone and teeth. 
In addition to the short account of the Protozoa given by 
