406 C. ClilFFOED DOBELL. 



distinct nucleus lji7ig towards the centre. Later (Vejdovsky, 

 1904), he describes stages in the mitotic division of this 

 nucleus, and records similar nuclei in certain filamentous 

 Bacteria inhabiting the gut oi: Bryodrilus ehlersi. 



Marpmaun (1900) suggests — amongst other things — that 

 enucleate Bacteria may exist. His observations are very 

 fragmentary, and all made upon flame-fixed organisms. 



Feinberg (1900) describes '' nuclei '' of various forms in 

 various species of Bacteria (B. coli, B. anthracis, Micro- 

 cocci, etc.). The observations were made upon organisms 

 stained by Romanowski's method. The method of fixation 

 is not given ; presumably the preparations were dried and 

 flame-fixed. (See here also Zettnow, 1900.) 



Marx and Woithe (1900) arrive at the conclnsion that the 

 Babes-Ernst granules aiford an index of virulence — greater 

 numbers indicating a greater degree of pathogenicity. They 

 further state that the organisms containing these granules 

 are the "Trager und Erhalter der Art." They also make the 

 statement that " the Babes-Ernst granules are products of 

 maximal condensation and typical localisation of the euchro- 

 matic substance of the bacterial celL" The illuminating 

 nature of such a statement is obvious. Regarding the relation 

 between the granules and virulence, the statement of Marx 

 and Woithe has been controvei-ted by Ascoli (1901), Grauss 

 (1902), Schumburg (1902), Krompecher (1901), Ficker (1903), 

 Guilliermond (1906), and others. 



Krompecher (1901), working on various organisms, draws 

 a distinction between "metachromatic grannies " and " Babes- 

 Ernst granules," on the grounds of staining reactions. He 

 leaves the significance of the graimles in doubt. (See here 

 also Muhlschlegel [1900], Marx [1902], etc.) 



Hinze (1901) found scattered granules, which he believed 

 to be chromatin, in the cells of Beggiatoa. Later (Hinze, 

 1903), he described simihir bodies in another large sulphur 

 bacterium — Thiophysa volutans. Various methods of 

 fixation (Flemming, etc.) and staining (Heidenhain, etc.) 

 were employed. The granules are said to divide by a process 



