CONTRIBUTIONS TO THE CYTOLOGY OF THE BACTERIA. 411 



"autogamy" of B. biitschlii (Scliandinn^ 1902) and B. 

 flexilis was probably not a sexual process at all. 



Arnato (1908) describes results obtained by staining several 

 Bacteria (B. mycoides^ Sp. volutaus, etc.) intra-vitani 

 with Brillantcresylblau. He believes that in the spore, and 

 at the beginning of development, a relatively large spherical 

 nucleus is present, which breaks up subsequently into cliro- 

 midia. The divergent views of different observers may have an 

 explanation in the fact that they observed similar oi-gauisms, 

 but at different stages in their development. 



Dangeard (1909) records the results of a i-e-investigation 

 of Chromatium. By fixing with Flemmiug or Perenyi, 

 and staining" with various stains (especially Flennning's 

 triple), he confirms the description of this organism given by 

 Biitschli. A "central body" corresponding to a nucleus is 

 present. Additional evidence for regarding the " central 

 body" as a nuclens is afforded by the fact that a rhizoplast 

 can sometimes be seen connecting the flagelluni with this body. 



Ambroz (1909) gives a lengthy description of Bacillus 

 nitri . As a result, he reaches the same conclusion as Ruzicka 

 — that Bacteria are nuclei. Fixation is said to have been 

 effected with a concentrated solution of "HCb,''^ and staining 

 chiefly with Giemsa. 



Under the name "Hillhousia" mirabilis, West and 

 Grifhths (1909) describe a very large sulphur bacterium. 

 There is said to be a protoplasmic network present, containing 

 granules believed not to be chromatin. "Nothing of the 

 natnre of a definite nuclens is present." Details — especially 

 as regards the method of using formol as a fixative — are too 

 scanty for this conclusion to be accepted without further 

 evidence. No reference is made to the work of Biitschli, 

 Schewiakoft', Hiuze and others, on similar forms. 



Recently, an account of the structure of the long forms of 

 B. coli, B. typhosus, i^tc. — produced by growing these 

 organisms on culture media containing aniline dyes — has been 



' I presume this means HgCl.,,— and not HCl, as given by GuiUier- 

 mcnd in a review of this paper in ' Bull. Inst. Pasteur.' 



VOL. 56, PART 3. NEW SERIES. 29 



