(IX cjasTisriKA veneris nov. spec. 529 



no chromatic border. It is totally different from the imdulating 

 luembraue of a Trypanqsome, to which most previous workers 

 have likened it. My interpretation of this characteristic 

 structure is the same as that of Gross (1910). "Die Crista 

 ist eiu Organell sui generis." 



A deeply staining ("chromatic ") edge to the crista and a 

 fibrillar structure can only be seen in macerated organisms^ 

 or organisms which have been imperfectly fixed. Such struc- 

 tuies must therefore be regarded as artifacts. The normal 

 crista of C. veneris stains pink or violet with Giemsa^s 

 stain, but this does not necessarily iudicate tliat it contains 

 chromatin. 



Flagella. — Flagella or cilia, such as occur in flagellate 

 Protozoa or Bacteria, are not present in Oris tispirag. The 

 matter has been fully discussed, bj^ Schellack (1909) and Gross 

 (1910), Avho have both come to this same conclusion. Further 

 discussion Avill therefore be superfluous. 



Protoplasmic Structure. — The chambered structrue of 

 the protoplasm, which I have described in C. veneris^ has 

 already been clearly recognised in other Cristispir^B by 

 Schellack and Gross. I am convinced^ with these two observers, 

 that the various nuclear figures (spiral filaments, transverse 

 rodlets, tetrads, etc.) described b}'^ Perrin and others are really 

 ai-tifacts. Moreover, Perrin\s account (1906) of the relations 

 existing between the various nuclear figures and the longi- 

 tudinal division of the organism must be discarded. For the 

 nuclear figures are artifacts, and longitudinal division does 

 not occur. 



The interpretations of the appearances observed by Schellack 

 and Gross differ from that which I have given in preceding- 

 pages. It will therefore be necessary to discuss their views 

 briefly. 



Schellack's (1909) interpretation of the protoplasmic 

 structure of Cristispira is somewhat similar to mine. 

 His description of the structure of the chambers is in close 

 agreement with my own description. In one point, however, 

 Schellack's interpretation differs from mine. He believes 



