( 860 ) 
normal animal treated likewise, we see that in the former a strong 
haemolysis has manifested itself, which in the latter is hardly percep- 
tible. The hypersensibility, therefore, of the organism is really — 
and that by the intervention of the anaphylactic guineapig-serum — 
localized in the red corpuscles, a fact by which a localization also 
upon other bodily cells is of course by no means excluded, on the 
contrary rendered probable. 
3v the side of this haemolysis we now meet with another pheno- 
menon. I have already pointed out before that the disappearance of 
alexine in vivo can be proved only in intraperitoneally intoxicated 
animals which do not die, or survive at least half an hour; in other 
words, to bring about this process some time is necessary. For 
guineapigs getting the second injection directly into the circulation *) 
and perishing within a few minutes show no loss of alexine. If, 
however, the blood of an animal treated thus, — defibrinated or 
not — is for some length of time left to itself in the incubator, 
the complement may, also under these circumstances, be seen to 
disappear. In the normal guineapig this process is hardly present, 
but in the sensitized animal it is much more distinct. This reaction, 
therefore, if commenced in vivo and set going, also spreads in vitro, 
only somewhat more slowly. 
Now the following question may be asked: does, while these 
phenomena are coming about, the serum of the hypersensitive animal 
play an active or a passive part? In order to study this question I 
was in a position to avail myself of an immunity-phenomenon — 
the so-called Conglutination-reaction — of which I may as well 
point out in a few words the origin and the signification. 
Enrricn and Sacus’) were at the time the first who drew attention 
to a peculiar reaction, which takes place between red corpuscles of 
the guineapig, fresh horse-serum and inactivated cattle-serum. Herein 
the erythrocytes show a peculiar conglomeration, which can be 
distinguished from the ordinary agglutinationtype (conglutination), 
followed by a haemolysis. The explanation of this phenomenon has 
led to a controversy between the schools of Eurrien and Borper 
about the action of amboceptors (sensibilisators) and complement 
(alexine) — a question with which we do not wish to meddle here. 
From the publications concerning this subject, by Borper and Gay *), 
+ 1 cM’. — The injection-tube with the serum has previously been brought 
to 57° 
