( 861 ) 
Sachs and Bauer *), Borper and Srrene*) (the last gave the pheno- 
menon the name of “Conglutinationreaction’’) it has meanwhile appeared 
that for the influence of the conglutinating cattle-serum on the 
guineapig-blood a previous fixation of the horse-alexine to this blood 
is necessary. Accordingly e.g. the relative complementary power of 
a horse-serum may in this way be easily determined. But besides it 
will also be clear that, if in some way or other this fixation of the 
horse-alexine to the guineapig-erythrocytes can be influenced (either 
furthered or checked), the course and the result of the whole process 
may thereby be altered. Now where the just mentioned elements 
play such an important part in the anaphylactic complex of symptoms, 
and where we wanted to trace the importance herefor of the serum 
of sensitized animals, it was also a matter of course to study the 
influence of such a serum — both before and after the toxic injection — 
on the course of the conglutinin-reaction. For it was to be expected 
a priori that the bodily fluids of the guineapigs specifically hyper- 
sensitive with respect to horse-serum also in reference to this serum 
would show some alterations in their qualities. 
To bring about the reaction discussed just now, we add together: 
*/,, cM.* fresh horse-serum with about the double volume (°/,,) of 
the guineapig-serum, then a little physiological solution of sodium- 
chlorid (°/;,). */2, ‘washed normal guineapig-blood and finally °*/,, 
cattle-serum (during ‘/, hour warmed to 55—56°). 
Merely through the presence of normal guineapig-serum the reaction 
is now somewhat retarded; but important for us is only the comparison 
of the course of the reaction in the tubes with the anaphylactic 
guineapig-serum before resp. after the toxic injection. In the main 
the following facts may be observed : 
After the intraperitoneal injection of a toxic dose the serum of 
sensitive guineapigs gets a strong antialexic power with regard to 
horse-serum, i.e.: it hinders the fixation of the horse-alexine on the 
guineapig-blood, which is necessary to bring about the conglutination- 
reaction. Now it is a well-known fact — which I pointed out in 
my previous communication — that a sensitized guineapig which 
remains alive after the second intraperitoneal serum-injection (and 
this depends only on the dose), is rendered immune against a following 
injection. It may therefore be surmised that the explanation of this fact 
lies in the just mentioned quality directed against the horse-serum, 
with which the serum of the animal defends the sensitive elements 
1) Arb. a. d. Königl. Inst. f. exp. Ther. zu Frankf. a. M. Heft 3, 1907, 
2) Centralbl f. Bakt. Bd. 49, 1909. 
