1258 
We have succeeded in obtaining plasma in both of these above- 
mentioned ways, first from the rabbit and afterwards from man, 
and in subsequently demonstrating in a direct chemical way the 
impermeability of the bloodcorpuscles towards glucose. 
Il. Determination of the amount of sugar in the bloodplasma 
of the rabbit obtained from a vein isolated from the body. 
To obtain blood-plasma from a blood-vessel our primary idea 
was that we could make use of the property of the bloodcorpuscles 
of female animals (especially pregnant ones) of settling rapidly 
compared to those of male animals’). Aceordingly we several times 
clamped the marginal vein of a she-rabbits ear which did not show 
apparent anastomoses, the rabbit being bound on a rabbit plank 
and the ear in question held vertically. We did not succeed, however, 
in obtaining sedimentation in this way, probably because, after all, 
there still existed small anastomoses on account of which the blood 
could still circulate in the clamped vein. 
By another method, however, the desired result was obtained 
with the same animals. 
Artuts *) has found that when blood is kept in a vein which is 
taken from the body and ligated at both ends, the blood remains 
fluid in this vein, and, what is of great importance with regard to 
the question under discussion, shows no glycolysis. This method for 
obtaining uncoagulable plasma has, practically speaking, up to the 
present been followed only with the jugular of the horse and is 
therefore known as the jugular-method. 
We have applied it twice to obtain pure plasma from rabbits. 
Here we set to work in the following way: The jugular on one 
side/ was laid open over a length of at least 4 cms. and dissected 
free from the neighbouring tissues and the greatest length between 
two of its confluent veins was doubly ligated at both ends. This 
part which was + 21!/, ems. long in both cases was then removed 
from the body and held vertically. Seeing that it would take too 
long to wait for the bloodeorpuscles to settle down when the vein 
was hung up we placed it in a small centrifugal tube in which 
the vein just reached to the bottom, and eentrifugalized rapidly. 
After some minutes there could be seen through the wall of the 
1) FäHRAEUS. Biochem. Zeitschr. 89. 355. 1918. 
*) ArrHus. Arch. de Physiologie 1891— 1892, 
