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vein the distinet division between the dark mass of bloodcorpuscles 
and the pale yellow translucent plasma. The bloodvessel was then 
ligated in the lowest layer of plasma and a prick hole made in 
the top portion from which the oozing plasma was caught up on 
two pieces of Bang’s paper. In both cases we obtained enough 
plasma to enable us to make a reliable double determination of the 
plasma sugar. Simultaneously with this blood was drawn from a 
vein in the ear of the same rabbit and in this the amount of blood 
sugar and the relative volume of blood corpuscles was determined. 
The result of both experiments was the following : 
a. b. 6 d. 
Total Volume Amount of plasma 
Plasmasugar. ploddétsar of sugar calculated 
Ba bloodcorpuscles.| from 6 and c. 
Exper. I. 0.266 0/, 0.194 9%, 27 0.2657 9/, 
ame | 0.255 » 0.1935 27 0.265 
We have therefore obtained with the jugular-method the result 
almost surprisingly accurate and accordanto that 7m the case of the 
rabbit the blood sugar occurs almost exclusively im the plasma. 
It can be remarked here still that both rabbits which were operated 
upon under a light ether anaesthesia showed a pronounced hypergly- 
caemia. This hyperglycaemia could therefore be reduced totally to 
a hyperglucoplasmia. 
UI. Zo show the impermeability of the bloodcorpyscles of man towards 
sugar by the paraffin method. 
To investigate also in the case of man the impermeability of the 
blood corpuscles towards sugar along directly chemical lines we 
first used the vein method for obtaining the plasma. Upon the 
advise of Prof. HAMBURGER the umbilical cord was used as human 
vein. Through the condencension of Prof. NisHorr and the house 
obstetricians of the obstetrical clinic in Groningen we had for some 
weeks at our disposal perfectly fresh umbilical cords. We tried 
repeatedly to bring about in pieces of umbilical cord a division 
between plasma and bloodcorpuscles in the large vein which could not 
always be traced distinctly, because this vein could only with great 
difficulty be dissected free from the neighbouring tissue with which 
onl 
