1261 
Total Sugar in the Volume of the Calculated 
bloodsugar. plasma. bloodcorpuscles. ih ee 
0.117 0/, 0.178 °/, 38 0/, 0.188 °/, 
0.103 0.161 39 0.169 
0.110 0.165 38 0.180 
0.135 0.223 43 0.237 
0.137 0.192 38 0.221 
0.111 0.188 41 0.190 
In our opinion these results afford a direct chemical proof that 
the bloodcorpuscles of man are free from sugar. 
This decision will have to be taken into account in clinical 
examinations so that besides the determination of the amount of 
sugar in the whole of the blood the volume of the bloodcorpuscles 
will have to be determined. 
The contradiction which we find with the many authors on this 
subject’) we hold has its origin in the following facts which we 
wil] return to in extenso later: 
1. Only in blood in which no signs of coagulation have appeared, 
we find the bloodcorpuscles free from glucose (hirudine and other 
substances which are supposed to make the blood uncoagulable, do 
not arrest the very first phases of the process of coagulation). 
2. The existence of a glucose-colloid-compound must be taken 
into account. 
3. Experiments which purpose the examination of the permeability 
of the bloodcorpuseles towards glucose with the aid of the intro- 
duction of fresh glucose must be judged with great care because the 
relative permeabilities of the «- and $-modifications of the a-glucose 
which result on solution of the latter are by no means equal ’). 
Groningen, December 1920. Physiological Laboratory. 
') See f.i. FALTA en RICHTER-QUITTNER. l.c. 
R. Ear. Biochem. Zeitschr. 111. 189. 1920. 
BONNIGER. ,, De 103. 306. 1920. 
BRINKMAN en v. DAM. Biochem. Zeitschr. 105. 93., 108. 74. 1920. 
HAGEDOORN. - En 107. 248. 1920. 
FEIGL. re 112. 54. 1920. 
M. B. WisHart. Journ. Biol. Chemistry. 44. 563. 1920. 
TacHAU. Zeitschr. Klin. Med. 79. 421. 1914. 
GRADWOHL and BLAivas. Journ. Lab. and Clin. Med. IL. No. 6. 1917. 
4) HAMBURGER. Proceedings of the Royal Acad. of Sciences XXI. N°. 4, XXVIII 
p. 318 and 327. | 
