1333 
suspension of amylum in NaCl-solution are added, which had likewise 
been treated with N, and the mixture thus obtained is exposed to 
the effect of body-temperature for 25 minutes. 
If, however, the same experiment was carried out in exactly the 
same manner, but only with this difference that the fluid treated 
with nitrogen could act at room-temperature for 5 hours instead of 
half an hour on the phagocytes, then the phagocytosis was found 
to be much less than in the original fluid, which had not been 
treated with nitrogen. Hence after a longer exposure of the phago- 
eytes to a medium which contains little oxygen, paralysis will set 
in, the available amount of oxygen being consumed to a great extent. 
This may appear from the following experiments. 
PA BLE, ME 
Effect of an extensive withdrawal of O on phagocytosis by a 
long exposure of the phagocytes to the normal medium 
and to the medium treated with N. 
| Phagocytosis 
After a 5 hours’ exposure of the 681 
phagocytes to the serous NaCl-sol. | — * 100 = 50.71°/, 
which had not been treated with N. | 1941 
After a 5 hours’ exposure of the 521 
phagocytes to the serous NaCl-sol. | 4-4 > 100 = 44.380/, 
which had been treated with N. 1174 
Whilst formerly after an exposure of one hour an increased 
phagocytosis was invariably observed, this increase has changed 
into a decrease after a 5 hours’ exposure. 
We shall add another experiment, showing the effect on the 
same leucocytes of an exposure of '/, hour and 4'/, hours. 
FABLE Vill. 
Effect of a short and of a long exposure of the phagocytes to 
a solution containing only traces of oxygen. 
Exposure of 1/2 hour | Exposure of 41/9 hours 
| 
In the ee serous In the normal serous 
44 
NaCl-sol.: 44 < 100 = 25.90, NaCl-sol. : os > 100=39.49/ 
941 
In the serous NaCl-sol. contain.|In the serous NaCl-sol. contain. 
2 
atrace of O: =! 100 = 34.59/o'a trace of O: ne X< 1003255 
930 
