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one? Or were the conditions not favourable for a thorough dust- 
emulsion of the whole mass of fat? 
In order to decide this question the uppermost layer (large drops 
of fat) was taken off by means of a pipette and shaken anew with 
fresh ascites-liquid. Centrifugal force was again applied and once 
more a separation into two layers was visible. Both lavers contained 
fat; now the undermost Jayer even contained more fat than at the 
beginning of the experiment and the microscope only showed the 
dustshape. From this it was evident, that the part of the fat, which, 
with the first experiment was separated in the shape of globules 
into an uppermost layer, had been transformed into fat in the shape 
of dust by shaking with fresh ascites-liquid. 
That which had not passed into dust was shaken again with 
fresh ascites-liquid, and now at length all the fat had been turned 
into the shape of dust. 
That with the first shaking-experiment the fat only partly passed 
into dustform, does not find its cause in an eventual difference in 
the relative condition of the different kinds of fat in the oil, but 
can be explained from the conditions of the experiment. It has in- 
deed been proved that a perfect dust-shaped emulsion can be obtained 
at once, if only the shaking is continued for a long time* and with 
a relatively large quantity of ascitestiquid. 
T have further considered whether a peculiar quality of the ascites- 
liquid must account for this. Therefore the experiment was repeated 
with another albuminous liquid, viz. with bloodserum. 30 ee. horse- 
serum were mixed with 5 cc. lipanine and the mixture strongly 
shaken for one hour. The emulsion was next centrifugalized, and 
thereby divided itself into two layers, a lower one with fat in dust- 
shape, an upper one with tiny fatglobules. : 
The latter was removed, vigorously shaken with 30 ce. of the 
fresh serum and after that centrifugalized again; once more two 
layers were obtained; the lower one however now contained much 
more fat than with the first shaking. After having been shaken 
third time with 30 ee. serum, all the fat was brought into the 
form of dust. Shaking 150 cc. serum with 5 cc. lipanine for four 
hours, brought about the perfect dust-shaped emulsion. This emulsion 
could now no more be divided into two layers by centrifugalizing. 
We thus did not find any specifie quality in our ascites-liquid with 
regard to the dispersion of the fat, for the same oceurred with the 
blood-serum. 
Transferring these facts to normal life — which does not seem 
too hazardous in this case — it can be imagined, that the lymph 
