( 433 ) 
By this we have proved, that by letting the air stream through the 
mixture of blood and lipanine-ascites-emulsion a considerable trans- 
formation of fat takes place. 
From (3) 0.371 gr. Ether residu is obtained 
vy (4) 0283 er. vv Ne 7} / 
From these two numbers it is evident that by letting a current 
of air pass through a mixture of blood and dust-shaped lipanine- 
serum-emuision, no transformation of fat takes place. 
These numbers even rather tend in the opposite direction. 
After all these experiments it must be taken for granted, that 
the lipolytic ferment was not present in the blood, nor in the serum, but 
existed in the ascites-liquid. 
The question could now be examined, whether the presence of blood is 
really required for the transformation of fat and whether it is not suf- 
ficient to pass air through the lipanine-ascites-emulsion. 
To reply to this question, 80 cc. of a lipanine-ascites-emulsion 
(75 ce. ascites-liquid + 5 ec. lipanine) were shaken for 3 hours 
and submitted to a current of air for 20 hours. After that the fat- 
contents were determined, which took place at the same time with 
a portion of the same emulsion, not having been treated with a 
current of air. 
80 cc. lipanine-ascites-emulsion treated with air contained 4.300 er. of fat 
80 ec. „ „ / not 4 e= / 4.252 wow wn 
The passing through of air only, has consequently not given cause 
to transformation of fat. 
This result agrees with that of COHNSTEIN and Mrcuaöris. These 
investigators also found in their experiments with true chyle, that 
without the presence of red blood-corpuscles, the passing through 
of a current of air was not able to cause a transformation of fat. 
Repetition of the experiment. 
This experiment was performed in the same manner as the fore- 
going; the only difference being that instead of 24 hours, the current 
of air was only allowed to pass for 12!/, hours, under room-tem- 
perature, and instead of horse-blood, ox-blood was used. 
(1) 75 ec. of dust-shaped lipanine-ascites-emulsion + 25 ee. oxeblood. Current 
of air for 121/, hours; after that the liquid is mixed with sand, dried an extracted 
with ether. Ether-extract 0.064 gr, 
